Center for Molecular Imaging, The Brown Foundation of Molecular Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA.
Annu Rev Med. 2012;63:217-31. doi: 10.1146/annurev-med-070910-083323. Epub 2011 Oct 27.
Technical developments in near-infrared fluorescence (NIRF) imaging and tomography have enabled recent translation into investigational human studies. Noninvasive imaging of the lymphatic vasculature for diagnosis and assessment of function has been uniquely accomplished with NIR using indocyanine green (ICG), a nonspecific dye that has comparatively poor fluorescent properties compared to emerging dyes. Adjunct use of NIRF-ICG for (a) intraoperative sentinel lymph node mapping for cancer staging, (b) video-angiography during surgery, and (c) discrimination of malignant from benign breast lesions detected by mammography and ultrasongraphy also evidences the clinical utility of NIRF. Future NIRF imaging agents that consist of bright fluorescent dyes conjugated to disease-targeting moieties promise molecular imaging and image-guided surgery. In this review, emerging NIRF imaging is described within the context of nuclear imaging technologies that remain the "gold standard" of molecular imaging.
近红外荧光 (NIRF) 成像和层析技术的发展使得其最近已被应用于人体研究。使用吲哚菁绿 (ICG) 进行的非侵入性淋巴管成像，可用于诊断和评估功能，这是 NIR 的独特应用，ICG 是一种非特异性染料，与新兴染料相比，其荧光特性相对较差。NIRF-ICG 的辅助应用包括：(a) 癌症分期的术中前哨淋巴结绘图，(b) 手术期间的血管造影，以及 (c) 通过乳腺 X 线摄影术和超声检测到的恶性和良性乳腺病变的区分，也证明了 NIRF 的临床效用。由与疾病靶向部分偶联的明亮荧光染料组成的未来 NIRF 成像剂有望实现分子成像和图像引导手术。在本综述中，新兴的 NIRF 成像在核成像技术的背景下进行了描述，核成像技术仍然是分子成像的“金标准”。
