Federal University of Lavras, Lavras 37200-000, MG, Brazil.
Res Vet Sci. 2012 Oct;93(2):733-5. doi: 10.1016/j.rvsc.2011.09.014. Epub 2011 Oct 28.
Population diversity was evaluated in strains of Staphylococcus aureus isolated from bovine mastitis in Brazilian dairy herds by PCR-RFLP and sequencing of the 3'-terminal portion of the coagulase gene, and the susceptibility of strains to antimicrobials. The results showed great diversity in S. aureus population studied and the existence of predominant clones that account for most infections. No associations between the predominant types observed in the PCR-RFLP and the forms of presentation of the mastitis or to any of the different patterns of antimicrobial resistance were observed.
采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和凝固酶基因 3'-末端序列分析的方法,评估了从巴西奶牛乳腺炎中分离的金黄色葡萄球菌菌株的种群多样性,并检测了菌株对各种抗生素的敏感性。结果表明,所研究的金黄色葡萄球菌种群具有高度多样性,存在主要克隆株,这些克隆株导致了大多数感染。在 PCR-RFLP 中观察到的主要类型与乳腺炎的表现形式或任何不同的抗生素耐药模式之间均无相关性。