Piastowska-Ciesielska Agnieszka W, Drobnik Jacek, Zarzyńska Joanna, Domińska Kamila, Russell John A, Ochędalski Tomasz
Department of Comparative Endocrinology, Medical University of Lodz, Poland.
Folia Histochem Cytobiol. 2011;49(3):497-503. doi: 10.5603/fhc.2011.0070.
Angiotensin II (AngII) is the biologically active peptide of the renin-angiotensin system (RAS). Tissue- based, local RAS has been identified in the prostate, testis, epididymis and coagulating glands. Experimental and clinical studies have consistently shown that myocardial infarction (MI) is associated with activation of the systemic RAS with increased concentration of angiotensin peptides in the blood and changes in expression of angiotensin receptors (AT). Changes in angiotensin receptors in the renal and cardiovascular system after MI are well recognized, but the effects of MI influence on changes in other tissue like the prostate gland are unknown. In the present study, we investigated the effect of myocardial infarction on angiotensin receptor protein and mRNA expression in the rat prostate gland. MI model was established in Wistar rats by ligating the left coronary artery (modified Selye method). The levels of AT1a-b and AT2 receptor mRNAs and proteins were measured in the rat prostate. Our study demonstrates tissue-specific changes in AT1a-b and AT2 receptor expression after myocardial infarction. The results show that MI has a strong influence on the expression of angiotensin receptor type AT1 in the prostate at the protein and mRNA level.
血管紧张素II（AngII）是肾素-血管紧张素系统（RAS）的生物活性肽。基于组织的局部RAS已在前列腺、睾丸、附睾和凝固腺中被发现。实验和临床研究一致表明，心肌梗死（MI）与全身RAS的激活有关，血液中血管紧张素肽浓度升高，血管紧张素受体（AT）表达发生变化。心肌梗死后肾和心血管系统中血管紧张素受体的变化已得到充分认识，但心肌梗死对前列腺等其他组织变化的影响尚不清楚。在本研究中，我们调查了心肌梗死对大鼠前列腺中血管紧张素受体蛋白和mRNA表达的影响。通过结扎左冠状动脉（改良的赛莱氏法）在Wistar大鼠中建立心肌梗死模型。测定大鼠前列腺中AT1a-b和AT2受体mRNA和蛋白水平。我们的研究表明心肌梗死后AT1a-b和AT2受体表达存在组织特异性变化。结果表明，心肌梗死在蛋白质和mRNA水平上对前列腺中AT1型血管紧张素受体的表达有强烈影响。
