Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, People's Republic of China.
Acta Biomater. 2012 Feb;8(2):866-77. doi: 10.1016/j.actbio.2011.10.020. Epub 2011 Oct 20.
Surface modification of titanium (Ti) using biomolecules has attracted much attention recently. In this study, a new strategy has been employed to construct a stable and bioactive coating on Ti. To this end, a derivative of hyaluronic acid (HA), i.e. HA-GRGDSPC-(SH), was synthesized. The disulfide-crosslinked Arg-Gly-Asp (RGD)-containing collagen/hyaluronic acid polyelectrolyte membrane (PEM) coating was then fabricated on Ti through the alternate deposition of collagen and HA-GRGDSPC-(SH) with five assembly cycles and subsequent crosslinking via converting free sulphydryl groups into disulfide linkages (RGD-CHC-Ti group). The assembly processes for PEM coating and the physicochemical properties of the coating were carefully characterized. The stability of PEM coating in phosphate-buffered saline solution could be adjusted by the crosslinking degree, while its degradation behaviors in the presence of glutathione were glutathione concentration dependent. The adhesion and proliferation of MC3T3-E1 cells were significantly enhanced in the RGD-CHC-Ti group. Up-regulated bone specific genes, enhanced alkaline phosphatase activity and osteocalcin production, the increased areas of mineralization were also observed in the RGD-CHC-Ti group. These results indicate that the strategy employed herein may function as an effective way to construct stable, RGD-containing bioactive coatings on Ti.
最近，利用生物分子对钛（Ti）进行表面改性引起了广泛关注。在这项研究中，采用了一种新策略在 Ti 上构建稳定且具有生物活性的涂层。为此，合成了透明质酸（HA）的衍生物，即 HA-GRGDSPC-（SH）。通过交替沉积胶原和 HA-GRGDSPC-（SH）进行五次组装循环，然后通过将游离巯基转化为二硫键（RGD-CHC-Ti 基团）进行交联，在 Ti 上制备了包含 Arg-Gly-Asp（RGD）的交联胶原/透明质酸聚电解质膜（PEM）涂层。仔细表征了 PEM 涂层的组装过程和涂层的物理化学性质。PEM 涂层在磷酸盐缓冲溶液中的稳定性可以通过交联度进行调节，而其在谷胱甘肽存在下的降解行为则取决于谷胱甘肽的浓度。RGD-CHC-Ti 组中 MC3T3-E1 细胞的黏附和增殖明显增强。RGD-CHC-Ti 组中还观察到骨特异性基因上调、碱性磷酸酶活性和骨钙素产生增强以及矿化面积增加。这些结果表明，本文所采用的策略可能是在 Ti 上构建稳定的、含有 RGD 的生物活性涂层的有效方法。
