Kyyak Solomiya, Pabst Andreas, Heimes Diana, Kämmerer Peer W
Department of Oral- and Maxillofacial Surgery, University Medical Center Mainz, 55131 Mainz, Germany.
Department of Oral- and Maxillofacial Surgery, Federal Armed Forces Hospital, 56072 Koblenz, Germany.
Materials (Basel). 2021 May 27;14(11):2885. doi: 10.3390/ma14112885.
Bovine bone substitute materials (BSMs) are used for oral bone regeneration. The objective was to analyze the influence of BSM biofunctionalization via hyaluronic acid (HA) on human osteoblasts (HOBs). BSMs with ± HA were incubated with HOBs including HOBs alone as a negative control. On days 3, 7 and 10, cell viability, migration and proliferation were analyzed by fluorescence staining, scratch wound assay and MTT assay. On days 3, 7 and 10, an increased cell viability was demonstrated for BSM+ compared with BSM- and the control (each ≤ 0.05). The cell migration was enhanced for BSM+ compared with BSM- and the control after day 3 and day 7 (each ≤ 0.05). At day 10, an accelerated wound closure was found for the control compared with BSM+/- (each < 0.05). The highest proliferation rate was observed for BSM+ on day 3 ( ≤ 0.05) followed by BSM- and the control (each ≤ 0.05). At day 7, a non-significantly increased proliferation was shown for BSM+ while the control was higher than BSM- (each < 0.05). The least proliferation activity was observed for BSM- ( < 0.05) at day 10. HA biofunctionalization of the BSMs caused an increased HOB activity and might represent a promising alternative to BSM- in oral bone regeneration.
牛骨替代材料（BSMs）用于口腔骨再生。目的是分析通过透明质酸（HA）对牛骨替代材料进行生物功能化处理对人成骨细胞（HOBs）的影响。将含有或不含HA的BSMs与HOBs一起培养，单独培养的HOBs作为阴性对照。在第3天、第7天和第10天，通过荧光染色、划痕试验和MTT试验分析细胞活力、迁移和增殖情况。在第3天、第7天和第10天，与不含HA的BSM和对照组相比，含HA的BSM（BSM+）显示出细胞活力增加（均P≤0.05）。在第3天和第7天后，与不含HA的BSM和对照组相比，含HA的BSM的细胞迁移增强（均P≤0.05）。在第10天，与含或不含HA的BSM相比，对照组的伤口愈合加快（均P<0.05）。在第3天，含HA的BSM观察到最高的增殖率（P≤0.05），其次是不含HA的BSM和对照组（均P≤0.05）。在第7天，含HA的BSM显示增殖无显著增加，而对照组高于不含HA的BSM（均P<0.05）。在第10天，不含HA的BSM观察到最低的增殖活性（P<0.05）。对BSMs进行HA生物功能化处理可提高HOB活性，在口腔骨再生中可能是不含HA的BSM的一种有前景的替代物。
