Laboratory of Biopathology and Molecular Biology, Av. Nenê Sabino, 1801, University of Uberaba, Uberaba, Minas Gerais 38055-500, Brazil.
Arch Oral Biol. 2012 May;57(5):469-76. doi: 10.1016/j.archoralbio.2011.10.011. Epub 2011 Oct 29.
In this study we investigated the possible use of human demineralised dentine matrix (DHDM), obtained from the extracted teeth, as bone graft material and evaluated the expression of vascular endothelial growth factor (VEGF) induced by this material in the healing process of tooth sockets of rats.
To evaluate bone regeneration and expression of VEGF induced by DHDM, thirty-two male Wistar rats weighing approximately 200 g were used. After maxillary second molar extraction, the left sockets were filled with DHDM and the right sockets were naturally filled by blood clot (control). The animals were sacrificed at 3, 7, 14 and 21 days after surgery and upper maxillaries were processed for histological, morphometric and immunohistochemical analyses. DHDM was used to evaluate the mechanical effect of bone graft material into sockets. Expression of VEGF was determined by immunohistochemistry in all groups.
Our results demonstrated a significant increase in the newly formed bone tissue in sockets of 7, 14 and 21 days and a significant increase in VEGF expression at days 7 and 14 on treated sockets.
Our results showed that DHDM increases the expression of VEGF and accelerates the healing process in rats tooth sockets, by stimulating bone deposition and also vessels formation. These results suggest that DHDM has osteoinductive/osteoconductive potential and may represent an efficient grafting material on guided bone regeneration.
本研究旨在探讨从拔牙中获得的人脱矿牙本质基质（DHDM）作为骨移植材料的可能用途，并评估该材料在大鼠牙槽骨愈合过程中诱导血管内皮生长因子（VEGF）的表达。
为了评估 DHDM 诱导的骨再生和 VEGF 的表达，我们使用了 32 只雄性 Wistar 大鼠，体重约 200 克。在拔除上颌第二磨牙后，左侧牙槽窝填充 DHDM，右侧牙槽窝自然填充血凝块（对照组）。术后 3、7、14 和 21 天处死动物，对上颌骨进行组织学、形态计量学和免疫组织化学分析。使用 DHDM 评估骨移植材料对牙槽窝的机械作用。所有组均通过免疫组织化学检测 VEGF 的表达。
我们的结果表明，在 7、14 和 21 天的牙槽窝中，新形成的骨组织明显增加，在处理后的牙槽窝中，VEGF 的表达在第 7 天和第 14 天也明显增加。
我们的结果表明，DHDM 通过刺激骨沉积和血管形成，增加 VEGF 的表达并加速大鼠牙槽骨的愈合过程。这些结果表明，DHDM 具有成骨/骨引导潜力，可能成为引导骨再生的有效移植材料。
