Department of Medicine and Aging Sciences, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
UdA-TechLab, Research Center, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Biomolecules. 2023 Nov 30;13(12):1727. doi: 10.3390/biom13121727.
Several studies have already demonstrated the biocompatibility of a tooth as a grafting material in the regeneration of bone tissue, showing its osteoconductive potential, while no studies have verified whether the osteoinductive potential of a tooth remains constant or is altered after its treatment with the Tooth Transformer (TT) device. The aim of the study was to demonstrate that the treatment with the TT device did not alter the osteoinductivity of an extracted tooth that was stored dry. Twelve extracted human teeth were collected from real patients. Caries, tartar and filling materials were removed from each tooth; each tooth was coarsely cut and stored at room temperature (RT) until use. Each sample was shredded, demineralized and disinfected, using the TT device. Protein extraction was carried out for each sample, and Western Blot analysis was performed to test the presence of mineralization protein LIM-1 and transforming growth factor-β. The presence of the human Bone Morphogenetic Protein 2 (BMP-2) and human collagen Type I (COL-I) was found in dry tooth samples processed with the TT device and subjected to Enzyme-Linked Immunosorbent Assay (ELISA) testing. The treatment of chemical demineralization using the TT device does not alter the osteoinductive potential of a dry tooth.
已有多项研究证实,牙齿作为一种移植材料在骨组织再生中具有生物相容性,显示出其成骨能力,而目前尚无研究证实牙齿的成骨诱导潜力在经过 Tooth Transformer(TT)设备处理后是否保持不变或发生改变。本研究旨在证明 TT 设备的处理不会改变干燥保存的拔牙的成骨诱导能力。从实际患者中收集了 12 颗人离体牙。从每颗牙上去除龋坏、牙石和填充材料;每颗牙都被粗切成块并在室温(RT)下储存,直到使用。对每个样本进行粉碎、脱矿和消毒,使用 TT 设备。对每个样本进行蛋白质提取,并进行 Western Blot 分析以检测矿化蛋白 LIM-1 和转化生长因子-β的存在。使用 TT 设备处理并进行酶联免疫吸附测定(ELISA)检测,发现干燥牙样本中存在人骨形态发生蛋白 2(BMP-2)和人胶原蛋白 I(COL-I)。使用 TT 设备进行化学脱矿处理不会改变干燥牙的成骨诱导能力。