Tang J C, Wong S F, Mak J W, Ho T M
International Medical University, 57000 Kuala Lumpur, Malaysia.
Trop Biomed. 2011 Aug;28(2):223-36.
House dust mites and storage mites are well-known causes for allergenic diseases. The aim of this study was to investigate the immunogenic sites of Blomia tropicalis, Aleurogyphus ovatus and Glycycometus malaysiensis. The mites were maintained in a culture medium at 25ºC and 75% relative humidity. Mites were harvested either with heat escape or floatation method, purified, homogenized, quantified and used for the production of polyclonal antibody and immunostaining. For each species of mites, five male mice and five male rats were randomly selected and immunized intraperitoneally with respective crude mite extract at two-weekly intervals. Blomia tropicalis, A. ovatus or G. malaysiensis whole mites and paraffin-embedded mite sections were immunostained with the respective polyclonal antibody. The faecal pellets of mites were intensely stained for all the three species in the present study. The legs of sectioned A. ovatus were not immunogenic as compared with those of G. malaysiensis and B. tropicalis. The outer layer (cuticle) of whole mites and the eggs for these species were very immunogenic. Hence, the polyclonal antibodies obtained in this study may serve as potential tools in detecting the eggs and immature mites in environmental samples. Future studies should focus on the antigenic components of eggs since they were relatively abundant in dust and highly antigenic as seen in the present study.
屋尘螨和仓储螨是过敏性疾病的常见病因。本研究旨在探究热带博氏无气门螨、卵形粉螨和马来西亚嗜甜螨的免疫原性位点。将螨饲养于温度25ºC、相对湿度75%的培养基中。采用热逃逸法或漂浮法收集螨，进行纯化、匀浆、定量，用于制备多克隆抗体和免疫染色。对于每种螨，随机选取5只雄性小鼠和5只雄性大鼠，每隔两周腹腔注射相应的粗螨提取物进行免疫。用各自的多克隆抗体对热带博氏无气门螨、卵形粉螨或马来西亚嗜甜螨的全螨及石蜡包埋的螨切片进行免疫染色。在本研究中，所有这三种螨的粪便颗粒均被强烈染色。与马来西亚嗜甜螨和热带博氏无气门螨相比，卵形粉螨切片的腿部无免疫原性。这些螨的全螨外层（角质层）和卵具有很强的免疫原性。因此，本研究中获得的多克隆抗体可能成为检测环境样本中螨卵和未成熟螨的潜在工具。鉴于在本研究中螨卵在灰尘中相对丰富且具有高度抗原性，未来的研究应聚焦于螨卵的抗原成分。
