Kort H S, Schober G, Koren L G, Scharringa J
Interuniversity Task Group Home and Health, Utrecht State University, The Netherlands.
Clin Exp Allergy. 1997 Aug;27(8):921-5.
Measurement of guanine in dust proved a good assessment of mite allergen exposure.
Exposure to mite allergens may lead to atopic inflictions. In a semi-natural test system the development of Dermatophagoides pteronyssinus (Trouessart) and Glycyphagus domesticus (De Geer), and the presence of their guanine excretion, was examined in a dust-soiled and mouldy environment. Mites were counted after heat-escape, and guanine was detected by means of capillary zone electrophoresis. For each species, 50 mites randomly taken, were inoculated on soiled test-surfaces of 10 x 10 cm. Rough wooden board, gypsum board, tufted carpet, and a self-made mattress representing wall surfaces and home-textiles, respectively, were used. Eight weeks after inoculation with mites only, the surfaces were all mould ridden, and mite and guanine measurements were taken. The Spearman rank correlation test and the Mann-Whitney U-test were used in statistical analysis. The confidence limit was set at 1%.
Among the various test-surfaces, no differences were found regarding total mite numbers and amount of guanine present (P > 0.01). For the dust-eating mite D. pteronyssinus, total mite numbers correlated with the amount of guanine present (P = 0.002) on all inoculated surfaces, indicating feeding on the protein-rich dust. For the mould devouring mite G. domesticus, however, no such correlation was found (P = 0.72). Apparently, they mainly consumed fungal carbohydrates during this experiment.
The allergological relevance of storage mites has been under discussion for the last 25 years. In humid homes, these mites will feed almost exclusively on fungi and may produce allergenic or irritating substances different from those arising on protein-rich laboratory media used in allergen extract production or present in carpets, bedding and furniture.
测定灰尘中的鸟嘌呤可很好地评估螨过敏原暴露情况。
接触螨过敏原可能导致特应性疾病。在一个半自然测试系统中，研究了粉尘螨（Trouessart）和腐食酪螨（De Geer）在灰尘污染且发霉环境中的生长情况及其鸟嘌呤排泄情况。螨经热逃逸后计数，鸟嘌呤通过毛细管区带电泳检测。对于每个物种，随机选取50只螨接种在10×10厘米的污染测试表面上。分别使用代表墙面和家用纺织品的粗糙木板、石膏板、簇绒地毯和自制床垫。仅接种螨8周后，所有表面都布满霉菌，然后进行螨和鸟嘌呤测量。统计分析采用Spearman秩相关检验和Mann-Whitney U检验。置信限设定为1%。
在各种测试表面中，螨总数和鸟嘌呤含量均无差异（P>0.01）。对于食尘螨粉尘螨，在所有接种表面上，螨总数与鸟嘌呤含量相关（P = 0.002），表明其以富含蛋白质的灰尘为食。然而，对于食霉菌螨腐食酪螨，未发现这种相关性（P = 0.72）。显然，在本实验中它们主要消耗真菌碳水化合物。
在过去25年里，仓储螨的变应原相关性一直存在争议。在潮湿的家庭中，这些螨几乎只以真菌为食，可能产生与变应原提取物生产中使用的富含蛋白质的实验室培养基上或地毯、床上用品和家具中存在的物质不同的变应原性或刺激性物质。
