Athens University Faculty of Nursing, ICU and Research Unit at KAT General Hospital 2, Nikis st GR, 14561 Athens, Greece.
Clin Exp Med. 2012 Dec;12(4):225-31. doi: 10.1007/s10238-011-0162-5. Epub 2011 Nov 1.
Immune system is crucial to tumour's initiation, progress and establishment and is contributing to prevent upcoming damaging invasion. Tumour development and surgical resection are both immunosuppressive processes. Immune response could be evaluated by ex vivo lipopolysaccharide (LPS) test, measuring cytokines and receptors release. The aim of the study is to investigate the postoperative immune recovery of cancer patients upon discharge. Twenty-two patients with colon cancer, without pre-treatment, and 16 healthy volunteers (HV) were enrolled in the study. Ten ml of whole blood were collected from every patient on admission (PRE) and upon discharge (POD7) and every HV. Diluted whole blood samples were stimulated with 500 pg/ml LPS, at 37°C, for 4H. Cell culture supernatants (CCSP) were removed after centrifugation and stored at -70°C. Tumour necrosis factor-alpha (TNF-α), interleukin-6 and interleukin-10 (IL-6, IL-10), soluble TNF receptors (sTNFRs) were measured in serum and CCSP by enzymelinked immunosorbent assay. Serum cytokines and receptors, PRE and POD7, were significantly elevated compared to HV (P < 0.001) and significant correlations were found between POD7 IL-6 and sTNFRs (Spearman's ρ 0.47, P < 0.05). Ex vivo, TNF-α, IL-6 and TNFRI release were lower either PRE or POD7, while IL-10 and TNFRII were higher, than in HV. No significant differences (P > 0.05) were found between PRE and POD7 levels in serum or CCSP. Cancer patients are not postoperatively immune restored until discharge. The trend of anti-inflammatory TNFRs release could account for alternative marker for the control of cancer patients immune response and the schedule of their following therapeutic treatment.
免疫系统对肿瘤的发生、发展和建立至关重要，并有助于防止即将发生的破坏性侵袭。肿瘤的发展和手术切除都是免疫抑制过程。可以通过体外脂多糖 (LPS) 试验来评估免疫反应，测量细胞因子和受体的释放。本研究旨在调查癌症患者出院后的术后免疫恢复情况。纳入了 22 名未经治疗的结肠癌患者和 16 名健康志愿者 (HV)。每位患者在入院时 (PRE) 和出院时 (POD7) 以及每位 HV 采集 10ml 全血。将稀释的全血样本在 37°C 下用 500pg/ml LPS 刺激 4 小时。离心后取出细胞培养上清液 (CCSP)，并储存在 -70°C。通过酶联免疫吸附试验测量血清和 CCSP 中的肿瘤坏死因子-α (TNF-α)、白细胞介素-6 和白细胞介素-10 (IL-6、IL-10)、可溶性 TNF 受体 (sTNFR)。与 HV 相比，血清细胞因子和受体 PRE 和 POD7 显著升高 (P < 0.001)，并且在 POD7 时发现 IL-6 和 sTNFRs 之间存在显著相关性 (Spearman's ρ 0.47，P < 0.05)。体外，TNF-α、IL-6 和 TNFRI 的释放无论是 PRE 还是 POD7 都较低，而 IL-10 和 TNFRII 则较高。在血清或 CCSP 中，PRE 和 POD7 之间的水平没有发现显著差异 (P > 0.05)。癌症患者直到出院后才恢复术后免疫。抗炎性 TNFRs 释放的趋势可能是控制癌症患者免疫反应和随后治疗方案的替代标志物。
