Wang K W, Han J S
Department of Physiology, Beijing Medical University, PRC.
Sci China B. 1990 Jun;33(6):686-93.
Behavioural studies suggested that cerebral angiotensin II (AII) plays an important role in the development of tolerance to electroacupuncture (EA) analgesia. Observations made in this study revealed an increase in AII immunoreactivity (AII-ir) in both CSF and brain as well as an increase in the cerebral content of AI-ir in rats rendered tolerant to EA. The extracts of brain from rats receiving EA for 1h and 3h were subjected to gel filtration and the elution profile was compared with that of normal brain extract. There was a marked right shift of the AII peak from the large molecule precursor to the small molecule AII-ir. The latter peak showed the same retention time in HPLC system as that of AII. The results suggest that the acceleration of the synthesis and release of AII during a long-term EA stimulation might constitute one of the mechanisms for EA tolerance.
行为学研究表明，脑内血管紧张素II（AII）在电针（EA）镇痛耐受性的形成中起重要作用。本研究中的观察结果显示，在对EA产生耐受性的大鼠中，脑脊液和脑内的AII免疫反应性（AII-ir）均增加，同时脑内AI-ir含量也增加。对接受1小时和3小时EA刺激的大鼠脑提取物进行凝胶过滤，并将洗脱图谱与正常脑提取物的洗脱图谱进行比较。AII峰从大分子前体明显右移至小分子AII-ir。后一个峰在高效液相色谱系统中的保留时间与AII相同。结果表明，长期EA刺激期间AII合成和释放的加速可能是EA耐受性的机制之一。
