[VEGF小干扰RNA诱导中国仓鼠肺成纤维细胞染色体畸变的研究]
[Research of chromosome aberration in Chinese hamster lung fibroblast induced by VEGF siRNA].
作者信息
Jing Chunxia, Zhang Huan, Yang Guang, Wu Chipeng, He Lin
机构信息
Department of Epidemiology, Medical College, Jinan University, Guangzhou 510632, China.
出版信息
Wei Sheng Yan Jiu. 2011 Sep;40(5):576-8.
OBJECTIVE
To explore the effects of VEGF siRNA on chromosome aberration in Chinese hamster lung fibroblasts (CHL).
METHODS
The chromosome aberrations were observed after CHL were transfected with VEGF siRNA for 24 hours and 48h.
RESULTS
There were a questionable positive in 100nmol/L VEGF siRNA after VEGF siRNA were transfected for 24h and the chromosome aberration rate was 6 percentages. Both 50nmol/L VEGF siRNA and 100nmol/L VEGF siRNA caused the questionable positive after VEGF siRNAs were transfected for 48h, and the chromosome aberration rates was 6 percentages and 10 percentages separately. There were no the chromosome aberration in 25nmol/L VEGF siRNA. The types of chromosomal aberration induced by VEGF siRNAs included break, Dicentric grain, polyploid, gap, and three trajectories.
CONCLUSION
100nmol/L VEGF siRNA might cause CHL chromosome aberration.
目的
探讨血管内皮生长因子（VEGF）小干扰RNA（siRNA）对中国仓鼠肺成纤维细胞（CHL）染色体畸变的影响。
方法
将VEGF siRNA转染CHL细胞24小时和48小时后，观察染色体畸变情况。
结果
VEGF siRNA转染24小时后，100nmol/L VEGF siRNA出现可疑阳性，染色体畸变率为6%。VEGF siRNA转染48小时后，50nmol/L和100nmol/L VEGF siRNA均出现可疑阳性，染色体畸变率分别为6%和10%。25nmol/L VEGF siRNA未出现染色体畸变。VEGF siRNA诱导的染色体畸变类型包括断裂、双着丝粒颗粒、多倍体、裂隙和三射体。
结论
100nmol/L VEGF siRNA可能导致CHL染色体畸变。
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