[亚硒酸钠对MNNG和MNU体外诱导的V79细胞染色体畸变的影响]

[Effect of sodium selenite on the chromosomal aberration of V 79 cells induced in vitro by MNNG and MNU].

作者信息

Chen Q G, Gao F Z, Ke Y, Hu G G, Liu X, Liu J, Luo X M

出版信息

Zhonghua Zhong Liu Za Zhi. 1987 Jan;9(1):33-5.

Abstract

Effect of sodium selenite on chromosomal aberration of V 79 cells induced by MNNG and MNU was studied. Na2SeO3 alone, at the concentration of 10(-7)-10(-4) M, increased the incidence of chromosomal aberration. However, Na2SeO3 at 10(-7)-10(-5) M, having been preincubated with the cells for 4 hours, could reduce the number of cells with chromosomal aberration induced by MNNG. Na2SeO3 at 10(-7)-10(-4) M inhibited mutagenic activity of chromosomal aberration induced by MNU. The same inhibition was observed even sodium selenite was added to the medium simultaneously with this carcinogen. The results indicate that sodium selenite alone, at the concentration range used in this experiment, is an aberration-inducing agent. But when combined with the carcinogen, anti-cancer effect is obtained.

摘要

研究了亚硒酸钠对N-甲基-N'-硝基-N-亚硝基胍（MNNG）和N-甲基-N-亚硝基脲（MNU）诱导的V79细胞染色体畸变的影响。单独的Na2SeO3在10^(-7)-10^(-4)M浓度下会增加染色体畸变的发生率。然而，10^(-7)-10^(-5)M的Na2SeO3与细胞预孵育4小时后，可减少MNNG诱导的染色体畸变细胞数量。10^(-7)-10^(-4)M的Na2SeO3可抑制MNU诱导的染色体畸变的诱变活性。即使亚硒酸钠与这种致癌物同时添加到培养基中，也观察到了相同的抑制作用。结果表明，在本实验所用的浓度范围内，单独的亚硒酸钠是一种诱畸变剂。但与致癌物联合使用时，可获得抗癌效果。