Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Vaccine. 2011 Dec 6;29(52):9611-7. doi: 10.1016/j.vaccine.2011.10.053. Epub 2011 Oct 30.
The aim of this study is to investigate whether the activity of inducing immune response of glycyrrhetinic acid (GA) could be enhanced after GA was encapsulated with liposome. Three hundred and fifty 14-day-old chickens were randomly assigned to 7 groups and vaccinated with Newcastle disease (ND) vaccine. Simultaneously, the chickens in experimental groups were injected with the glycyrrhetinic acid liposome (GAL) at three doses, GA and blank liposome, respectively. The activity of serum antibody titer, concentrations of immunoglobulins G (IgG) and immunoglobulins M (IgM), lymphocytes proliferation, the proportions of lymphocyte subpopulations (CD4(+) and CD8(+)) was determined after vaccination. GAL was evaluated for inducing humoral immunity and cellular immunity in chicken against Newcastle disease (ND) vaccine. The results showed that GAL not only could significantly enhance the antibody titers, IgG and IgM in ND vaccine immunized chicken, but also significantly promote the lymphocyte proliferation and the proportions of CD4(+) and CD8(+), as comparison with GA, BL and VC control groups. Moreover, the effects appear a dose-dependent manner and a time-dependent manner. These indicated that GAL could significantly promote the activation potential of humoral immunity and cellular immunity in chicken and present certain dose-effect and time-effect relationships. The formulations of GA and liposome can further enhance the immune response against ND vaccine compared with the adjuvant alone.
本研究旨在探讨将甘草次酸（GA）包封于脂质体后，其诱导免疫应答的活性是否增强。将 350 只 14 日龄的鸡随机分为 7 组，用新城疫（ND）疫苗进行免疫接种。同时，实验组的鸡分别注射三次甘草次酸脂质体（GAL）、GA 和空白脂质体。免疫接种后，测定血清抗体效价、免疫球蛋白 G（IgG）和免疫球蛋白 M（IgM）浓度、淋巴细胞增殖、淋巴细胞亚群（CD4(+)和 CD8(+))比例。评估 GAL 对鸡抗新城疫（ND）疫苗的体液免疫和细胞免疫的诱导作用。结果表明，与 GA、BL 和 VC 对照组相比，GAL 不仅能显著提高 ND 疫苗免疫鸡的抗体效价、IgG 和 IgM，还能显著促进淋巴细胞增殖和 CD4(+)和 CD8(+)的比例。此外，这些效果呈现出剂量依赖性和时间依赖性。这表明 GAL 能显著促进鸡的体液免疫和细胞免疫的激活潜能，并呈现出一定的剂量效应和时间效应关系。与单独使用佐剂相比，GA 和脂质体的制剂能进一步增强对 ND 疫苗的免疫应答。
