O-连接的N-乙酰葡糖胺修饰的蛋白质的检测与分析
Detection and analysis of proteins modified by O-linked N-acetylglucosamine.
作者信息
Zachara Natasha E, Vosseller Keith, Hart Gerald W
机构信息
The Johns Hopkins University School of Medicine, Baltimore, Maryland.
Drexel University College of Medicine, Philadelphia, Pennsylvania.
出版信息
Curr Protoc Protein Sci. 2011 Nov;Chapter 12:12.8.1-12.8.33. doi: 10.1002/0471140864.ps1208s66.
Abstract
O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.
摘要
O-连接的N-乙酰葡糖胺（O-GlcNAc）是一种常见的对细胞核、线粒体及细胞质蛋白进行的翻译后修饰，它与II型糖尿病和阿尔茨海默病的病因以及心脏保护作用有关。本单元涵盖了用于鉴定被O-GlcNAc修饰的蛋白、研究添加和去除O-GlcNAc的酶以及定位O-GlcNAc修饰位点的简单和综合技术。
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Am J Physiol Heart Circ Physiol. 2010 Nov;299(5):H1715-27. doi: 10.1152/ajpheart.00337.2010. Epub 2010 Sep 10.
2
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3
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