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O-连接的N-乙酰葡糖胺修饰的蛋白质的检测与分析

Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

作者信息

Zachara Natasha E, Vosseller Keith, Hart Gerald W

机构信息

The Johns Hopkins University School of Medicine, Baltimore, Maryland.

Drexel University College of Medicine, Philadelphia, Pennsylvania.

出版信息

Curr Protoc Protein Sci. 2011 Nov;Chapter 12:12.8.1-12.8.33. doi: 10.1002/0471140864.ps1208s66.

DOI:10.1002/0471140864.ps1208s66
PMID:22045558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3349994/
Abstract

O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.

摘要

O-连接的N-乙酰葡糖胺（O-GlcNAc）是一种常见的对细胞核、线粒体及细胞质蛋白进行的翻译后修饰，它与II型糖尿病和阿尔茨海默病的病因以及心脏保护作用有关。本单元涵盖了用于鉴定被O-GlcNAc修饰的蛋白、研究添加和去除O-GlcNAc的酶以及定位O-GlcNAc修饰位点的简单和综合技术。

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OGA inhibition by GlcNAc-selenazoline.GlcNAc-硒唑啉抑制 OGA。
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Quantification of O-glycosylation stoichiometry and dynamics using resolvable mass tags.使用可分辨质量标签定量 O-糖基化化学计量和动态变化。
Nat Chem Biol. 2010 Sep;6(9):645-51. doi: 10.1038/nchembio.412. Epub 2010 Jul 25.
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Amino Acids. 2011 Mar;40(3):765-79. doi: 10.1007/s00726-010-0645-9. Epub 2010 Jun 19.
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