Tada Y, Yamaguchi J
Department of Bacteriology, Kinki University School of Medicine, Osaka, Japan.
FEMS Microbiol Lett. 1990 Jun 15;58(1):115-8. doi: 10.1016/0378-1097(90)90112-4.
Easily solubilized major outer membrane protein was found in Serratia marcescens. The protein was originally obtained as a membrane-associated, insoluble protein in the outer membrane when the cells were slightly disrupted. However, the amount of this protein in the outer membrane gradually decreased with the time of sonication. The decrease was not due to decomposition of the protein but to solubilization into a soluble fraction after a long period of disruption. The molecular weight of this protein was 47 kDa and it bound calcium. Another 40 kDa calcium binding protein was also found in the outer membrane of S. marcescens.
在粘质沙雷氏菌中发现了易溶解的主要外膜蛋白。最初，当细胞受到轻微破坏时，该蛋白是作为外膜中与膜相关的不溶性蛋白获得的。然而，外膜中这种蛋白的量随着超声处理时间的延长而逐渐减少。这种减少不是由于蛋白质的分解，而是由于长时间破坏后溶解到了可溶性部分中。这种蛋白的分子量为47 kDa，并且它能结合钙。在粘质沙雷氏菌的外膜中还发现了另一种40 kDa的钙结合蛋白。
