Tada Y, Yamaguchi J
Department of Bacteriology, Kinki University School of Medicine, Osaka, Japan.
Microbiol Immunol. 1994;38(3):171-5. doi: 10.1111/j.1348-0421.1994.tb01761.x.
The function of one of the outer membrane proteins of Serratia marcescens was investigated. S. marcescens with an abundant 40 kDa outer membrane protein was induced to form spheroplast at a high rate in an isotonic medium in the presence of calcium, although the spheroplasts were generally fragile in the isotonic environment. The degree of spheroplast induction was correlated to the amount of the 40 kDa protein present in the membrane. In the 40 kDa proteinless mutant strains, the spheroplast induction rate was remarkably decreased. Autoradiography of the outer membrane revealed the presence of a calcium-binding protein as a radioactive band whose position coincided with the 40 kDa protein. These results suggest that the 40 kDa protein has an important role in maintaining the structural integrity of the cell wall against osmotic shock.
对粘质沙雷氏菌一种外膜蛋白的功能进行了研究。在存在钙的等渗培养基中，具有丰富的40 kDa外膜蛋白的粘质沙雷氏菌被诱导以高比率形成原生质球，尽管原生质球在等渗环境中通常很脆弱。原生质球诱导程度与膜中存在的40 kDa蛋白的量相关。在无40 kDa蛋白的突变菌株中，原生质球诱导率显著降低。外膜的放射自显影显示存在一种钙结合蛋白作为放射性条带，其位置与40 kDa蛋白一致。这些结果表明，40 kDa蛋白在维持细胞壁抵抗渗透压冲击的结构完整性方面具有重要作用。
