Bar-Ness R, Rosenberg M
Maurice and Gabriela Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
J Gen Microbiol. 1989 Aug;135(8):2277-81. doi: 10.1099/00221287-135-8-2277.
Serratia marcescens RZ has been previously shown to possess pronounced cell-surface hydrophobicity, as evidenced by its affinity for hydrocarbons and polystyrene. The present report suggests the involvement of a 70 kDa protein, serraphobin, in this phenomenon. The 70 kDa protein was recovered from both the cell surface and culture supernatant of hydrophobic wild-type cells, but was either totally absent or present in minor quantities in hydrophobicity-deficient mutants. Similarly, loss of hydrophobicity of RZ cells following growth at 39 degrees C was accompanied by loss of the protein. Serraphobin was capable of binding to hexadecane droplets following a brief mixing procedure, and could be desorbed by solidifying and melting the hexadecane phase.
粘质沙雷氏菌RZ先前已被证明具有显著的细胞表面疏水性，这可通过其对碳氢化合物和聚苯乙烯的亲和力得到证明。本报告表明一种70 kDa的蛋白质——疏菌素，参与了这一现象。这种70 kDa的蛋白质可从疏水性野生型细胞的细胞表面和培养上清液中回收，但在疏水性缺陷型突变体中要么完全不存在，要么含量很少。同样，RZ细胞在39℃生长后疏水性的丧失伴随着该蛋白质的丧失。疏菌素在经过短暂混合程序后能够与十六烷液滴结合，并且可以通过固化和熔化十六烷相将其解吸。
