Institute of Oncology, Davidoff Center, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel.
World J Gastroenterol. 2011 Sep 21;17(35):3976-85. doi: 10.3748/wjg.v17.i35.3976.
To compare the microRNA (miR) profiles in the primary tumor of patients with recurrent and non-recurrent gastric cancer.
The study group included 45 patients who underwent curative gastrectomies from 1995 to 2005 without adjuvant or neoadjuvant therapy and for whom adequate tumor content was available. Total RNA was extracted from formalin-fixed paraffin-embedded tumor samples, preserving the small RNA fraction. Initial profiling using miR microarrays was performed to identify potential biomarkers of recurrence after resection. The expression of the differential miRs was later verified by quantitative real-time polymerase chain reaction (qRT-PCR). Findings were compared between patients who had a recurrence within 36 mo of surgery (bad-prognosis group, n = 14, 31%) and those who did not (good-prognosis group, n = 31, 69%).
Three miRs, miR-451, miR-199a-3p and miR-195 were found to be differentially expressed in tumors from patients with good prognosis vs patients with bad prognosis (P < 0.0002, 0.0027 and 0.0046 respectively). High expression of each miR was associated with poorer prognosis for both recurrence and survival. Using miR-451, the positive predictive value for non-recurrence was 100% (13/13). The expression of the differential miRs was verified by qRT-PCR, showing high correlation to the microarray data and similar separation into prognosis groups.
This study identified three miRs, miR-451, miR-199a-3p and miR-195 to be predictive of recurrence of gastric cancer. Of these, miR-451 had the strongest prognostic impact.
比较复发性和非复发性胃癌患者原发肿瘤中的微小 RNA（miRNA）谱。
研究组纳入了 45 名患者，他们于 1995 年至 2005 年期间接受了根治性胃切除术，且未接受辅助或新辅助治疗，且有足够的肿瘤含量。从福尔马林固定石蜡包埋的肿瘤样本中提取总 RNA，保留小 RNA 部分。使用 miRNA 微阵列进行初始分析，以鉴定切除后复发的潜在生物标志物。随后通过定量实时聚合酶链反应（qRT-PCR）验证差异 miRs 的表达。将 36 个月内手术后复发的患者（不良预后组，n=14，31%）和未复发的患者（良好预后组，n=31，69%）的发现进行比较。
在预后良好的患者和预后不良的患者的肿瘤中，发现 3 种 miRNA，miR-451、miR-199a-3p 和 miR-195 表达差异（P<0.0002、0.0027 和 0.0046）。每种 miRNA 的高表达均与复发和生存的不良预后相关。使用 miR-451，非复发的阳性预测值为 100%（13/13）。通过 qRT-PCR 验证了差异 miRNA 的表达，结果显示与微阵列数据高度相关，并且相似地分为预后组。
本研究鉴定了 3 种 miRNA，miR-451、miR-199a-3p 和 miR-195，可预测胃癌的复发。其中，miR-451 具有最强的预后影响。
