Department of Biology, Washington University, St. Louis, Missouri 63130, USA.
Int J Phytoremediation. 2011;13 Suppl 1:77-89. doi: 10.1080/15226514.2011.568537.
The combination of rational protein engineering and directed evolution techniques allow for the redesign of enzymes with tailored properties for use in environmental remediation. This review summarizes current molecular methods for either altering or improving protein function and highlights examples of how these methods can address bioremediation problems. Although much of the protein engineering applied to environmental clean-up employs microbial systems, there is great potential for and significant challenges to translating these approaches to plant systems for phytoremediation purposes. Protein engineering technologies combined with genomic information and metabolic engineering strategies hold promise for the design of plants and microbes to remediate organic and inorganic pollutants.
理性的蛋白质工程和定向进化技术的结合，可以对酶进行重新设计，使其具有特定的性质，用于环境修复。本文综述了当前改变或改善蛋白质功能的分子方法，并举例说明了这些方法如何解决生物修复问题。虽然应用于环境清理的蛋白质工程大部分采用微生物系统，但将这些方法转化为植物系统用于植物修复具有很大的潜力和挑战。蛋白质工程技术与基因组信息和代谢工程策略相结合，有望用于设计植物和微生物来修复有机和无机污染物。
