Diaconu Minodora, Diaconu C, Tarţău Liliana, Lupuşoru Cătălina-Elena
Universitatea de Medicină şi Farmacie "Gr. T. Popa" Iaşi, Facultatea de Medicină.
Rev Med Chir Soc Med Nat Iasi. 2011 Jul-Sep;115(3):876-80.
to study the effects of licht/dark induced-stress in an animal model of HELLP syndrome.
The experiment was carried out on white female Wistar rats treated intraperitoneally, for 4 weeks, as follows: Group 1: saline solution 0.5ml/100g weight; Group 2: saline solution + dark induced-stress, continuously, 28 days; Group 3: saline solution + light induced-stress, continuously, 28 days; Group 4: endotelin-l; Group 5: endotelin-1 + dark induced-stress, continuously, 28 days; Group 6: endotelin-1 + light induced-stress, continuously, 28 days. Endotelin-1 0.125nmol/kbw was initially administered, followed by 0.25nmol/kbw 30 min later, 2 times/week.
At the end of the experiment blood pressure was measured and blood samples were taken to assess: leukocyte formula, phagocytic capacity of peripheral neutrophils and serum complement activity, aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT) and lactic dehydrogenase (LDH) activity, total bilirubin and lipid profile.
In these experimental conditions we obtained an animal model of partial HELLP syndrome (elevated liver enzyme levels and a low platelet count, values emphasized by light induced-stress, without hemolysis). In chronically stressed animals we found the highest levels of blood pressure, a decrease of plasmatic cortisol level and of phagocytic capacity of peripheral neutrophils and an increase of total cholesterol.
研究光照/黑暗诱导应激对HELLP综合征动物模型的影响。
对白色雌性Wistar大鼠进行腹腔注射，持续4周，具体分组如下：第1组：0.5ml/100g体重的生理盐水；第2组：生理盐水+持续28天的黑暗诱导应激；第3组：生理盐水+持续28天的光照诱导应激；第4组：内皮素-1；第5组：内皮素-1+持续28天的黑暗诱导应激；第6组：内皮素-1+持续28天的光照诱导应激。最初给予0.125nmol/kbw的内皮素-1，30分钟后给予0.25nmol/kbw，每周2次。
实验结束时测量血压并采集血样以评估：白细胞计数、外周中性粒细胞的吞噬能力和血清补体活性、天冬氨酸转氨酶（ASAT）、丙氨酸转氨酶（ALAT）和乳酸脱氢酶（LDH）活性、总胆红素和血脂谱。
在这些实验条件下，我们获得了部分HELLP综合征的动物模型（肝酶水平升高和血小板计数降低，光照诱导应激使这些值更明显，无溶血）。在慢性应激动物中，我们发现血压水平最高，血浆皮质醇水平和外周中性粒细胞吞噬能力降低，总胆固醇升高。
