Diaconu C, Tarţău Liliana, Lupuşoru Cătălina-Elena
Universităţii de Medicină şi Farmacie Gr. T. Popa Iaşi, Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi, Facultatea de Medicină.
Rev Med Chir Soc Med Nat Iasi. 2011 Apr-Jun;115(2):349-53.
to investigate the influence of some stress factors in endothelin-1-induced hypertension.
The experiment was carried out on Wistar rats, treated intraperitoneally, for 4 weeks, as follows: Group 1: saline solution (0.5 ml/100 g weight); Group 2: saline solution + stress; Group 3: Endotelin-1; Group 4: Endotelin-l + stress; Group 5: Endotelin-1 + cholesterol diet (0.2 g/kbw/day); Group 6: Endotelin-1 + cholesterol diet + stress. Endotelin-1 0.25 nmol/kbw was initially administered, followed by 0.5 nmol/kbw 15 min later, 2 times/week. Stress-inducing factors were immobilization and water immersion. In the 28th day of the experiment blood pressure was measured and blood samples were taken from the retro-orbitary plexus to assess plasma cortisol, blood count, phagocytic capacity of peripheral neutrophils, and serum complement activity.
Repeated administration of endotelin-1 determined an increase in blood pressure, statistically significant in stress conditions comparing to non-stressed animals. Our study proved a decrease of plasma cortisol, total leukocyte count, phagocytic capacity of peripheral neutrophils, without significant alterations in serum complement activity.
Chronic exposure to complex stress conditions in rats with endothelin-1-induced hypertension determined a decrease of plasma cortisol levels, effect correlated with elevated blood pressure and decrease in the number and phagocytic function of peripheral neutrophils.
研究某些应激因素在内皮素 -1 诱导的高血压中的作用。
实验在 Wistar 大鼠身上进行，腹腔注射给药，持续 4 周，具体如下：第 1 组：生理盐水（0.5 ml/100 g 体重）；第 2 组：生理盐水 + 应激；第 3 组：内皮素 -1；第 4 组：内皮素 -1 + 应激；第 5 组：内皮素 -1 + 胆固醇饮食（0.2 g/kbw/天）；第 6 组：内皮素 -1 + 胆固醇饮食 + 应激。最初给予内皮素 -1 0.25 nmol/kbw，15 分钟后再给予 0.5 nmol/kbw，每周 2 次。应激诱导因素为固定和水浸。在实验的第 28 天测量血压，并从眶后丛采集血样，以评估血浆皮质醇、血细胞计数、外周中性粒细胞的吞噬能力和血清补体活性。
重复给予内皮素 -1 导致血压升高，与非应激动物相比，在应激条件下具有统计学意义。我们的研究证明血浆皮质醇、总白细胞计数、外周中性粒细胞的吞噬能力降低，而血清补体活性无明显改变。
在内皮素 -1 诱导的高血压大鼠中，长期暴露于复杂应激条件下会导致血浆皮质醇水平降低，这种效应与血压升高以及外周中性粒细胞数量和吞噬功能降低相关。
