Cernescu Irina Teodora, Tarţău Liliana, Macavei Antonela, Lupuşoru Cătălina-Elena
Universităţii de Medicină şi Farmacie "Gr. T. Popa" Iaşi.
Rev Med Chir Soc Med Nat Iasi. 2011 Oct-Dec;115(4):1195-9.
To investigate the effects of a Cetraria islandica extract in an animal model of hepatopathy.
The experiments were carried out on Wistar rats, treated intraperitoneally (excepting carbon tetrachloride which was administered orally by esogastric tube), at single daily dose, for 28 days and divided in eight groups of 6 rats each: Group 1: Saline solution (0.5ml/100g bw/day); Group 2: MgSO4 (5mg/kbw/day); Group 3: C. islandica (Ci) extract (21.56mg/kbw/day); Group 4: Ci+MgSO4; Group 5: carbon tetrachloride (CT).(0.1 ml/100g bw/day); Group 6: CT+MgSO4; Group 7: CT+Ci; Group 8: CT+Ci+MgSO4. At the end of the experiment blood samples were taken to assess the effects on the blood parameters,TGO, TGP, GGT, LDH, serum total billirubin, neutrophil phagocytic function (Nitroblue tetrazolium test) and serum complement activity. This parameters were determined with VITROS 750 XRC device, using Johnson&Johnson kits. The results were statistically processed with the help of "t-Student" test. p<0.05 was considered statistically significant.
In this carbon tetrachloride-induced hepatopathy animal model, C. islandica extract demonstrated hepatoprotective and immuno-stimulating effects (proved by normalization of glutamic-pyruvic transminase, glutamic-oxalocetic transminase, serum total billirubin, stimulation of neutrophils percentage and phagocytic functions, decreasing the carbon tetrachloride-induced basophilia and monocytosis, especially in association with magnesium).
Further studies are required to confirm the benefits of this association in hepatoprotective and immunomodulating therapies.
研究冰岛衣提取物在肝病动物模型中的作用。
实验在Wistar大鼠上进行，除四氯化碳经食管胃管口服给药外，其余均每日腹腔注射给药，持续28天，分为8组，每组6只大鼠：第1组：生理盐水（0.5ml/100g体重/天）；第2组：硫酸镁（5mg/kg体重/天）；第3组：冰岛衣（Ci）提取物（21.56mg/kg体重/天）；第4组：Ci + 硫酸镁；第5组：四氯化碳（CT）（0.1ml/100g体重/天）；第6组：CT + 硫酸镁；第7组：CT + Ci；第8组：CT + Ci + 硫酸镁。实验结束时采集血样，以评估对血液参数、谷丙转氨酶（TGO）、谷草转氨酶（TGP）、γ-谷氨酰转肽酶（GGT）、乳酸脱氢酶（LDH）、血清总胆红素、中性粒细胞吞噬功能（硝基蓝四氮唑试验）和血清补体活性的影响。这些参数使用强生试剂盒通过VITROS 750 XRC设备测定。结果采用“t检验”进行统计学处理。p<0.05被认为具有统计学意义。
在这个四氯化碳诱导的肝病动物模型中，冰岛衣提取物表现出肝脏保护和免疫刺激作用（通过谷丙转氨酶、谷草转氨酶、血清总胆红素的正常化得到证实，中性粒细胞百分比和吞噬功能受到刺激，四氯化碳诱导的嗜碱性粒细胞增多和单核细胞增多减少，特别是与镁联合使用时）。
需要进一步研究以证实这种联合在肝脏保护和免疫调节治疗中的益处。
