Solnica Bogdan, Skupien Jan, Kusnierz-Cabala Beata, Slowinska-Solnica Krystyna, Witek Przemyslaw, Cempa Agnieszka, Malecki Maciej T
Department of Diagnostics, Jagiellonian University Medical College, Krakow, Poland.
Clin Chem Lab Med. 2011 Nov 3;50(2):361-5. doi: 10.1515/CCLM.2011.770.
The aim of the study was to evaluate the effect of hematocrit (HCT) on glucose meter assays based on different measurement techniques.
This paper studied glucose meters utilizing the glucose dehydrogenase reaction and four measurement techniques: colorimetry (HemoCue), reflectometry (Accu-Chek Active), amperometry (Optium Xido) and coulometry (Optium Omega). The EDTA venous blood samples HCT were modified by adding or removing defined aliquots of plasma. Glucose concentration was measured using each meter in 27 batches of blood samples, with HCT ranging from 20% to 60% in 10% increments. The data were analyzed using repeated measures models and a linear random effects model.
A significant relationship between HCT and glucose reading in all meters was found and, for all meters except Optium Xido, there was a significant modification of this relationship by glucose level. The relative decrease in glucose concentration per 1% increase of the HCT value varied from 0.30% for Optium Omega in samples with glucose concentrations <5.55 mmol/L to 1.37% for Optium Xido in the same stratum (p<0.0001). The 5% glucose meter error (the ADA recommendation) was reached in the <5.55 mmol/L stratum after HCT change by 3.9%-16.7%.
There is a significant continuous effect of HCT on measurement accuracy of glucose meters across its wide range of values and glucose concentrations. The most sensitive to the HCT interference was the system utilizing amperometric technique (Optium Xido) followed by the one with reflectometric technique (Accu-Chek Active), while the systems with the coulometric technique (Optium Omega) or colorimetric measurements in whole blood haemolysate (HemoCue) were less sensitive.
本研究的目的是评估血细胞比容（HCT）对基于不同测量技术的血糖仪检测结果的影响。
本文研究了利用葡萄糖脱氢酶反应的血糖仪以及四种测量技术：比色法（HemoCue）、反射光度法（Accu-Chek Active）、安培法（Optium Xido）和库仑法（Optium Omega）。通过添加或去除特定体积的血浆来改变乙二胺四乙酸（EDTA）静脉血样本的HCT。使用各血糖仪对27批血液样本进行葡萄糖浓度测量，HCT范围为20%至60%，以10%的增幅递增。采用重复测量模型和线性随机效应模型对数据进行分析。
发现所有血糖仪的HCT与葡萄糖读数之间均存在显著关系，并且除Optium Xido外，所有血糖仪的这种关系均因葡萄糖水平而有显著改变。HCT值每增加1%，葡萄糖浓度的相对降低幅度在葡萄糖浓度<5.55 mmol/L的样本中，Optium Omega为0.30%，在同一分层中Optium Xido为1.37%（p<0.0001）。在HCT变化3.9% - 16.7%后，<5.55 mmol/L分层达到了5%的血糖仪误差（美国糖尿病协会（ADA）推荐值）。
HCT在其广泛的数值和葡萄糖浓度范围内对血糖仪的测量准确性有显著的持续影响。对HCT干扰最敏感的是采用安培技术的系统（Optium Xido），其次是采用反射光度技术的系统（Accu-Chek Active），而采用库仑技术的系统（Optium Omega）或全血溶血产物比色测量的系统（HemoCue）则较不敏感。
