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A mutant of human immunodeficiency virus with reduced RNA packaging and abnormal particle morphology.

作者信息

Clavel F, Orenstein J M

机构信息

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

出版信息

J Virol. 1990 Oct;64(10):5230-4. doi: 10.1128/JVI.64.10.5230-5234.1990.

DOI:10.1128/JVI.64.10.5230-5234.1990
PMID:2204725
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248025/
Abstract

A deletion of 30 bases was created in the noncoding region of the human immunodeficiency virus type 1 genome that extends between the 5' splice donor and the start of the gag gene. Viral particles produced after transfection of this mutant provirus had a normal protein pattern but a reduced RNA content. The infectivity of the mutant virus was also markedly reduced but not completely abolished. Electron-microscopic examination of the mutant virions revealed major abnormalities of the nucleoid structure, mostly related to the dense material characteristic of mature particles, suggesting that the presence of RNA is essential to the normal structure of the nucleoid.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/9de89a713a4f/jvirol00065-0641-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/795c51e84e67/jvirol00065-0639-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/9b212f5538a8/jvirol00065-0640-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/17dd5d79aac8/jvirol00065-0640-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/9de89a713a4f/jvirol00065-0641-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/795c51e84e67/jvirol00065-0639-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/9b212f5538a8/jvirol00065-0640-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/17dd5d79aac8/jvirol00065-0640-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a40d/248025/9de89a713a4f/jvirol00065-0641-a.jpg

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本文引用的文献

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Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.逆转录病毒包装突变体的构建及其用于产生无辅助病毒的缺陷型逆转录病毒。
Cell. 1983 May;33(1):153-9. doi: 10.1016/0092-8674(83)90344-6.
2
Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene.禽逆转录病毒脾坏死病毒的包装序列位于5'长末端重复序列和gag基因起始位点之间。
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5986-90. doi: 10.1073/pnas.79.19.5986.
3
Transfer of defective avian tumor virus genomes by a Rous sarcoma virus RNA packaging mutant.
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Elife. 2023 Jan 23;12:e83548. doi: 10.7554/eLife.83548.
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Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 years.用人类免疫缺陷病毒载体递送基因：25 年后仍处于最先进水平。
J Biomed Sci. 2022 Oct 9;29(1):79. doi: 10.1186/s12929-022-00865-4.
5
Sequestering the 5'-cap for viral RNA packaging.将 5'-帽结构隔离用于病毒 RNA 包装。
Bioessays. 2022 Nov;44(11):e2200104. doi: 10.1002/bies.202200104. Epub 2022 Sep 13.
6
Human Retrovirus Genomic RNA Packaging.人类逆转录病毒基因组 RNA 包装。
Viruses. 2022 May 19;14(5):1094. doi: 10.3390/v14051094.
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RNA Structures and Their Role in Selective Genome Packaging.RNA 结构及其在选择性基因组包装中的作用。
Viruses. 2021 Sep 8;13(9):1788. doi: 10.3390/v13091788.
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NMR Studies of Retroviral Genome Packaging.NMR 研究逆转录病毒基因组包装。
Viruses. 2020 Sep 30;12(10):1115. doi: 10.3390/v12101115.
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