SIAB Research, Gold Coast, Queensland, Australia.
J Clin Pathol. 2012 Jan;65(1):8-13. doi: 10.1136/jclinpath-2011-200269. Epub 2011 Nov 2.
Full blood counts are now used as evidence that athletes have used banned blood doping. This has led to legal scrutiny of the efficacy of preanalytical procedures such as specimen homogenisation.
To characterise the impact of different mixing strategies on whole-blood homogeneity.
Manual inversion, mechanical mixing and automatic mixing performed by the Sysmex XT-2000i were evaluated.
Automated mixing by the instrument, or 1 min of mechanical mixing, thoroughly homogenised specimens even for tubes that had been refrigerated and left undisturbed for 36 h. Manual inversions were almost as effective, provided that specimens were first allowed to equilibrate to ambient temperatures.
Current sport guidelines that mandate at least 15 min of mechanical mixing are excessive. Except where specimens are presented to the instrument manually, mechanical mixing is redundant in the context of full blood counts.
全血计数现在被用作运动员使用禁用血液兴奋剂的证据。这导致对标本均化等分析前程序的功效进行了法律审查。
描述不同混合策略对全血均一性的影响。
评估手动颠倒、机械混合和 Sysmex XT-2000i 自动混合。
即使对于冷藏并静置 36 小时的试管，仪器的自动混合或 1 分钟的机械混合也能彻底混合标本。手动颠倒也非常有效，只要标本首先允许平衡到环境温度。
目前要求至少 15 分钟机械混合的运动指南是过度的。除非标本手动送到仪器上，否则在全血计数的情况下，机械混合是多余的。
