Robinson Neil, Giraud Sylvain, Schumacher Yorck Olaf, Saugy Martial
Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Geneva and Lausanne, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Ch. des Croisettes 22, 1066, Epalinges, Switzerland.
Aspetar Orthopedic and Sports Medicine Hospital, Doha, Qatar.
Drug Test Anal. 2016 Feb;8(2):199-207. doi: 10.1002/dta.1804. Epub 2015 Apr 29.
Some recent studies have characterized the stability of blood variables commonly measured for the Athlete Biological Passport. The aim of this study was to characterize the impact of different shipments conditions and the quality of the results returned by the haematological analyzer. Twenty-two healthy male subjects provided five EDTA tubes each. Four shipment conditions (24, 36, 48, 72 h) under refrigerated conditions were tested and compared to a set of samples left in the laboratory also under refrigerated conditions (group control). All measurements were conducted using two Sysmex XT-2000i analyzers. Haemoglobin concentration, reticulocytes percentage, and OFF-score numerical data were the same for samples analyzed just after collection and after a shipment under refrigerated conditions up to 72 h. Detailed information reported especially by the differential (DIFF) channel scatterplot of the Sysmex XT-2000i indicated that there were signs of blood deterioration, but were not of relevance for the variables used in the Athlete Biological Passport. As long as the cold chain is guaranteed, the time delay between the collection and the analyses of blood variables can be extended.
最近的一些研究已经描述了运动员生物护照中常用血液变量的稳定性。本研究的目的是描述不同运输条件以及血液分析仪返回结果的质量所产生的影响。22名健康男性受试者每人提供了5支乙二胺四乙酸（EDTA）管。测试了冷藏条件下的四种运输条件（24、36、48、72小时），并与同样保存在实验室冷藏条件下的一组样本（对照组）进行比较。所有测量均使用两台Sysmex XT - 2000i分析仪进行。血红蛋白浓度、网织红细胞百分比和OFF评分数值数据在采集后立即分析的样本以及冷藏条件下运输长达72小时后的样本中是相同的。Sysmex XT - 2000i特别通过差分（DIFF）通道散点图报告的详细信息表明，存在血液变质的迹象，但与运动员生物护照中使用的变量无关。只要冷链得到保证，血液变量采集与分析之间的时间延迟就可以延长。
