He Shu-hua, Wei An-yang, Ye Ting-yu, Yang Yong, Luo Xin-gui, Liu Yang, Zhang Tao
Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
Zhonghua Nan Ke Xue. 2011 Oct;17(10):913-7.
To explore the effect of the calcitonin gene related peptide (CGRP) on the phenotypic transformation of corpus cavernosum smooth muscle cells (CCSM) in diabetic rats with erectile dysfunction (ED).
Models of diabetes and diabetic ED were established in male Sprague-Dawley rats by administration of streptozotocin, and CCSMs were primarily cultured and subjected to immunocytochemical assay. The cells were divided into a diabetic ED and a normal control group, and exposed to 0, 10, 60 and 100 nmol/L of CGRP for 24 hours. Then the relative expressions of calponin 1 (Cnn1) and osteopontin (OPN) mRNA were determined by real-time fluorescence quantitative RT-PCR (qRT-PCR).
The rate of SMalpha-actin positive cells in the CCSMs was (95.94 +/- 0.03) %. The expression of Cnn1 mRNA was significantly lower while that of OPN mRNA remarkably higher in the diabetic ED rats (4.41 +/- 0.29 and 5.28 +/- 0.32) than in the normal controls (10.35 +/- 0.62 and 1.32 +/- 0.24) (P < 0.01). Exposure to 100 nmol/L of CGRP significantly upregulated the expression of Cnn1 mRNA and downregulated that of OPN mRNA as compared with the unexposed rats (6.9 +/- 0.22 vs 4.41 +/- 0.29 and 3.26 +/- 0.31 vs 5.28 +/- 0.32, P < 0.01).
CGRP can transform the phenotype of CCSMs in diabetic ED rats from contractile to synthetic type.
探讨降钙素基因相关肽（CGRP）对糖尿病性勃起功能障碍（ED）大鼠阴茎海绵体平滑肌细胞（CCSM）表型转化的影响。
通过腹腔注射链脲佐菌素建立雄性Sprague-Dawley大鼠糖尿病及糖尿病性ED模型，原代培养CCSM并进行免疫细胞化学检测。将细胞分为糖尿病性ED组和正常对照组，分别给予0、10、60和100 nmol/L的CGRP处理24小时。然后采用实时荧光定量逆转录聚合酶链反应（qRT-PCR）检测钙调蛋白1（Cnn1）和骨桥蛋白（OPN）mRNA的相对表达量。
CCSM中平滑肌肌动蛋白（SMalpha-actin）阳性细胞率为（95.94±0.03）%。糖尿病性ED大鼠Cnn1 mRNA表达显著低于正常对照组（4.41±0.29 vs 10.35±0.62），OPN mRNA表达显著高于正常对照组（5.28±0.32 vs 1.32±0.24）（P<0.01）。与未处理组相比，100 nmol/L CGRP处理显著上调Cnn1 mRNA表达，下调OPN mRNA表达（6.9±0.22 vs 4.41±...
