Department of Medical Phamacology, Università degli Studi di Milano, Milan, Italy.
J Biol Regul Homeost Agents. 2011 Apr-Jun;25(2 Suppl):S35-42.
It is well known that the surface properties of biomaterials may affect bone-healing processes by modulating both cell viability and osteogenic differentiation. In this study we evaluated proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs) cultured on three prototypes of titanium disks and on thin layers of silicon carbide (SiC-PECVD), a material characterized by a high hardness and wear resistance. Our data indicated that all the tested surfaces supported cell growth, in particular, hASCs seeded on both titanium treated by a double-step etching process (TIT) and titanium modified by two Anodic Spark Deposition processes (TAA) grew better respect to the ones cultured on titanium obtained by KOH alkali etching process on TAA (TAAK). Furthermore, hASCs well colonized SiC-PECVD surface, showing a quite similar viability to cells cultured on plastic (PA). TIT and TAA better supported osteogenic differentiation of hASCs compared to PA, as shown by a marked increase of both alkaline phosphatase activity and calcified extracellular matrix deposition; in contrast TAAK did not positively affect hASCs differentiation. SiC-PECVD did not alter osteogenic differentiation of hASC cells: indeed, ALP and calcium deposition levels were comparable to those of cells cultured on plastic. Furthermore, we observed similar results testing hASCs either pre-differentiated for 14 days in osteogenic medium or directly differentiated on biomaterials. Our study suggests that modifications of titanium surface may improve osteo-integration of implant devices and that SiC-PECVD may represent a valid alternative for the coating of prosthetic devices to reduce wear and metallosis events.
众所周知，生物材料的表面特性可以通过调节细胞活力和成骨分化来影响骨愈合过程。在这项研究中，我们评估了在三种钛盘原型和碳化硅（SiC-PECVD）薄层上培养的人脂肪来源干细胞（hASC）的增殖和成骨分化，SiC-PECVD 是一种具有高硬度和耐磨性的材料。我们的数据表明，所有测试的表面都支持细胞生长，特别是在经过双步蚀刻处理（TIT）和经过两次阳极火花沉积处理（TAA）的钛上接种的 hASC 比在经过 KOH 碱蚀刻处理的 TAA 上获得的钛（TAAK）上培养的细胞生长得更好。此外，hASC 很好地定植在 SiC-PECVD 表面上，其活力与在塑料（PA）上培养的细胞相当。与 PA 相比，TIT 和 TAA 更能支持 hASC 的成骨分化，碱性磷酸酶活性和矿化细胞外基质沉积均明显增加；相比之下，TAAK 不会对 hASC 分化产生积极影响。SiC-PECVD 不会改变 hASC 细胞的成骨分化：事实上，ALP 和钙沉积水平与在塑料上培养的细胞相当。此外，我们在成骨培养基中预分化 14 天或直接在生物材料上分化的 hASC 上观察到类似的结果。我们的研究表明，钛表面的修饰可以改善植入物的骨整合，SiC-PECVD 可能是减少磨损和金属中毒事件的假体涂层的有效替代物。
