Benazzo Francesco, Botta Laura, Scaffino Manuela Federica, Caliogna Laura, Marullo Matteo, Fusi Simonetta, Gastaldi Giulia
Department of Orthopedics and Traumatology, IRCCS Policlinico San Matteo Foundation, University of Pavia, Italy.
J Biomed Mater Res A. 2014 Jul;102(7):2061-71. doi: 10.1002/jbm.a.34875. Epub 2013 Jul 30.
Trabecular Titanium (TT) is an innovative highly porous structure that imitates the morphology of trabecular bone with good mechanical properties. Adipose-derived stem cells are a multipotent cell population that can be used in regenerative medicine, in particular, for bone therapeutic applications. The ability of TT to induce the osteogenic differentiation of human adipose derived stem cells (hASCs) in the absence of osteogenic factors was evaluated using molecular biological, biochemical, and immunohistochemical methods. At 7 and 21 days from differentiation, the hASCs grown on TT scaffolds showed similar expressions of alkaline phosphatase (ALP) and Runx-2 both in the presence and in the absence of osteogenic factors, as well as at transcript and protein levels. hASCs cultured on monolayer in the presence of the medium obtained from the wells where hASCs/scaffold constructs were cultured in the absence of osteogenic factors differentiated towards the osteogenic phenotype: their gene and protein expression of ALP and Runx-2 was similar to that of the same cells cultured in the presence of osteogenic factors, and significantly higher than that of the ones cultured in growth medium.
小梁钛（TT）是一种创新的高孔隙结构，它模仿小梁骨的形态，具有良好的力学性能。脂肪来源干细胞是一种多能细胞群体，可用于再生医学，特别是骨治疗应用。使用分子生物学、生物化学和免疫组织化学方法评估了TT在无成骨因子情况下诱导人脂肪来源干细胞（hASCs）成骨分化的能力。在分化7天和21天时，在TT支架上生长的hASCs在有无成骨因子的情况下，以及在转录和蛋白质水平上，碱性磷酸酶（ALP）和Runx-2的表达均相似。在无成骨因子条件下培养hASCs/支架构建体的孔中获得的培养基存在时，单层培养的hASCs向成骨表型分化：它们的ALP和Runx-2基因和蛋白质表达与在有成骨因子条件下培养的相同细胞相似，且显著高于在生长培养基中培养的细胞。
