Department of Pathology, Chhatrapati Shahuji Maharaj Medical University, Lucknow 226003, Uttar Pradesh, India.
Oral Oncol. 2012 Mar;48(3):233-9. doi: 10.1016/j.oraloncology.2011.10.003. Epub 2011 Nov 2.
The aim of the study was to investigate whether the estimation of circulating Vascular endothelial growth factor-A (VEGF-A) levels by ELISA could be used as surrogate of VEGF-A expression in tissues of pre-malignant oral lesions (PMOLs) and oral squamous cell carcinoma (OSCC) as compared to that in healthy controls. The study samples comprised of tissue and blood samples from 60 PMOLs, 60 OSCC, and 20 healthy controls. Serum VEGF-A levels were determined by an ELISA based assay (Quantikine human VEGF; R & D System, Minneapolis USA). Tissue VEGF-A expression and microvessel density (MVD) were assessed by immunohistochemistry (IHC) using antibodies against VEGF-A and CD-34 on formalin fixed paraffin embedded (FFPE) tissue sections. VEGF-A mRNA expression was analyzed by real-time PCR in snap frozen tissues. Serum VEGF-A levels and immunohistochemical VEGF-A expression were significantly high in PMOLs and OSCC in comparison with controls. VEGF mRNA gene expression showed more than 50-fold increase in PMOLs and OSCC. VEGF-A levels in serum correlated in a linear fashion with the tissue expression in oral pre-malignant and malignant lesions, suggesting that the serum levels may serve as surrogate material for tissue expression of VEGF-A.
本研究旨在探讨与健康对照组相比，通过 ELISA 测定循环血管内皮生长因子 A (VEGF-A) 水平是否可作为癌前口腔病变 (PMOL) 和口腔鳞状细胞癌 (OSCC) 组织中 VEGF-A 表达的替代物。研究样本包括 60 例 PMOL、60 例 OSCC 和 20 例健康对照组的组织和血液样本。采用 ELISA 试剂盒（Quantikine 人 VEGF；R & D System，美国明尼苏达州）测定血清 VEGF-A 水平。采用针对 VEGF-A 和 CD-34 的抗体通过免疫组织化学 (IHC) 评估组织 VEGF-A 表达和微血管密度 (MVD)，在福尔马林固定石蜡包埋 (FFPE) 组织切片上进行。使用实时 PCR 在速冻组织中分析 VEGF-A mRNA 表达。与对照组相比，PMOL 和 OSCC 中的血清 VEGF-A 水平和免疫组化 VEGF-A 表达明显升高。PMOL 和 OSCC 中的 VEGF mRNA 基因表达增加了 50 多倍。血清中 VEGF-A 水平与口腔癌前和恶性病变中的组织表达呈线性相关，表明血清水平可能可作为 VEGF-A 组织表达的替代物。
