Li Qinglin, Fu Xiaoxuan, Ge Xinyang, Tao Feng, Huang Ping, Ge Minghua, Jin Hongchuan
Sir Run Run Shaw Hospital, Medical School of Zhejiang University, Hangzhou, China.
Zhejiang Cancer Hospital, Hangzhou, China.
Front Oncol. 2019 Jul 4;9:578. doi: 10.3389/fonc.2019.00578. eCollection 2019.
L. belongs to the genus and has a long history to be used as a Chinese medicinal herb to reduce swelling, control body temperature, and promote detoxification. However, its anticancer activity and mechanisms of action have not been evaluated yet. In the present study, we used several cell lines and xenograft models from different cancers to demonstrate the broad-spectrum anticancer activity of L as well as its underlying mechanisms of action and . The ethyl acetate extract of L showed good anticancer activity and was further fractioned to obtain five active components, including PEA to PEE. Among these fractions, PEC showed the strongest cytotoxicities against various cancer cell lines. It was further observed that PEC inhibited cancer cell growth, arrested cells at G2 phase, and induced apoptosis and suppressed tumor growth and angiogenesis in a dose- and time-dependent manner. Furthermore, PEC decreased the expression of vascular endothelial growth factor (VEGF) and micro-vascular density (MVD) in tumor tissues . It also promoted the proliferation of T and B lymphocytes, increased the activities of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), enhanced the secretion of interleukin 2 (IL-2) by spleen cells, and raised the levels of IgG, IgG2a, and IgG2b antibodies in tumor-bearing mice , which were at least partially responsible for the anticancer activity of PEC. In summary, PEC has shown broad-spectrum anticancer activities without causing any host toxicity and and may be developed as a preventive and therapeutic agent against human cancer. Further studies are urgently needed to determine the anticancer compounds in PEC and their detailed molecular mechanisms.
L. 属于该属，作为一种中草药用于消肿、控制体温和促进排毒已有很长历史。然而，其抗癌活性和作用机制尚未得到评估。在本研究中，我们使用了来自不同癌症的几种细胞系和异种移植模型，以证明L. 的广谱抗癌活性及其潜在的作用机制。L. 的乙酸乙酯提取物显示出良好的抗癌活性，并进一步分离得到五种活性成分，包括PEA至PEE。在这些馏分中，PEC对各种癌细胞系表现出最强的细胞毒性。进一步观察到，PEC抑制癌细胞生长，使细胞停滞在G2期，并诱导凋亡，且以剂量和时间依赖性方式抑制肿瘤生长和血管生成。此外，PEC降低了肿瘤组织中血管内皮生长因子（VEGF）的表达和微血管密度（MVD）。它还促进了T和B淋巴细胞的增殖，增加了自然杀伤（NK）细胞和细胞毒性T淋巴细胞（CTLs）的活性，增强了脾细胞分泌白细胞介素2（IL-2）的能力，并提高了荷瘤小鼠中IgG、IgG2a和IgG2b抗体的水平，这些至少部分是PEC抗癌活性的原因。总之，PEC已显示出广谱抗癌活性，且不会引起任何宿主毒性，可能被开发成为一种针对人类癌症的预防和治疗药物。迫切需要进一步研究以确定PEC中的抗癌化合物及其详细的分子机制。
