Roberts T A, Hudson W R, Whelehan O P, Simonsen B, Olgaard K, Labots H, Snijders J M, Van Hoof J, Debevere J, Dempster J F, Devereux J, Leistner L, Gehra H, Gledel J, Fournaud J
Meat Research Institute, Agricultural and Food Research Council, Langford, Bristol, Great Britain.
Meat Sci. 1984;11(3):191-205. doi: 10.1016/0309-1740(84)90037-8.
In seven member countries of the European Communities, three abattoirs were visited on three occasions in each of two surveys and at each visit ten beef carcasses were sampled, before chilling, at defined sites on the neck, brisket, forerib and medially on the round. In Survey I, samples were plated for total viable count (TVC) at 30° (ISO 2293) and Enterobacteriaceae at 37° (ISO 5552); in Survey II only TVCs were made. This paper is confined to analyses of the TVCs in the two surveys. Data from each country were analysed separately as sampling methodology may not have been sufficiently reproducible by different workers to allow between-countries comparison. Variations among visits to particular abattoirs and abattoir × site interactions made comparisons among abattoirs invalid within five of the seven countries. To effectively monitor differences between abattoirs within most countries it would be necessary to make more than three visits to each abattoir. Despite abattoir × site interactions in three countries in Survey I and four countries in Survey II, comparisons between sites were generally valid because of the consistent high contamination of the brisket. In the remainder of countries the abattoir × site interaction was too large to allow valid comparisons between sites. It is recommended that at least three or four sites are sampled in future surveys as only one site per carcass would underestimate the number of more heavily contaminated carcasses.
在欧洲共同体的七个成员国中,在两次调查的每次调查中,都对三个屠宰场进行了三次走访,每次走访时,在颈部、胸部、前肋骨和后腿内侧的规定部位,对十具牛胴体在冷藏前进行采样。在调查I中,样本接种于30℃用于测定总活菌数(TVC)(ISO 2293),接种于37℃用于测定肠杆菌科细菌数(ISO 5552);在调查II中,只测定了TVC。本文仅限于对两次调查中的TVC进行分析。由于不同工作人员的采样方法可能无法充分再现,无法进行国家间比较,因此对每个国家的数据分别进行分析。在七个国家中的五个国家,特定屠宰场不同走访之间的差异以及屠宰场×部位的相互作用使得屠宰场之间的比较无效。为了有效监测大多数国家内不同屠宰场之间的差异,有必要对每个屠宰场进行三次以上的走访。尽管在调查I中有三个国家以及调查II中有四个国家存在屠宰场×部位的相互作用,但由于胸部的污染一直很高,部位之间的比较通常是有效的。在其余国家中,屠宰场×部位的相互作用太大,无法进行部位之间的有效比较。建议在未来的调查中至少对三到四个部位进行采样,因为每具胴体只采一个部位会低估污染更严重的胴体数量。