Colello Rocío, Ruiz María J, Padín Valeria M, Rogé Ariel D, Leotta Gerardo, Padola Nora Lía, Etcheverría Analía I
Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET-CICPBA, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina.
Servicio Antígenos y Antisueros, Instituto Nacional de Producción de Biológicos, Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
Front Microbiol. 2018 Jun 28;9:1370. doi: 10.3389/fmicb.2018.01370. eCollection 2018.
The aim of the present study was to determine the prevalence of in the pork production chain and to characterize isolates. From 764 samples, 35 (4.6%) were positive for spp., as determined by biochemical tests and the presence of the gene. From these, 2.6, 2.0, 8.8, and 8.0% corresponded to samples collected from farms, slaughterhouses, boning rooms and retail markets, respectively. strains were classified into five serotypes and distributed as follows: Typhimurium in the pork production chain, . Kentucky in farms and slaughterhouses, . Brandenburg in slaughterhouses. Livingstone in farms and . Agona in boning rooms and retail markets. Interestingly, the antimicrobial susceptibility testing indicated that all 35 spp.-positive isolates were resistant to at least one antimicrobial agent, and 30 were multidrug-resistant (MDR) and resistant to different classes of antibiotics. The enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis showed clonal relatedness among strains isolated from farms, boning rooms and retail markets. The presence of antibiotic-resistant in food poses a potential health hazard to consumers.
本研究的目的是确定猪肉生产链中[具体细菌名称未给出]的流行情况，并对[具体细菌名称未给出]分离株进行特征描述。通过生化试验和[具体基因名称未给出]基因的存在情况，在764份样本中，有35份（4.6%）对[具体细菌名称未给出]属呈阳性。其中，分别有2.6%、2.0%、8.8%和8.0%的样本来自农场、屠宰场、去骨间和零售市场。[具体细菌名称未给出]菌株被分为五种血清型，分布如下：猪肉生产链中的鼠伤寒[具体细菌名称未给出]，农场和屠宰场中的肯塔基[具体细菌名称未给出]，屠宰场中的勃兰登堡[具体细菌名称未给出]，农场中的利文斯通[具体细菌名称未给出]，以及去骨间和零售市场中的阿哥纳[具体细菌名称未给出]。有趣的是，抗菌药敏试验表明，所有35份[具体细菌名称未给出]属阳性分离株均对至少一种抗菌剂耐药，30份为多重耐药（MDR）且对不同类别的抗生素耐药。肠杆菌重复基因间共识聚合酶链反应（ERIC-PCR）分析显示，从农场、去骨间和零售市场分离的菌株之间存在克隆相关性。食品中存在抗生素耐药性的[具体细菌名称未给出]对消费者构成潜在健康危害。
