Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Anal Biochem. 2012 Feb 1;421(1):103-7. doi: 10.1016/j.ab.2011.10.017. Epub 2011 Oct 15.
A new chiral biosensor has been fabricated by immobilizing γ-globulin on gold nanoparticles modified glassy carbon electrodes, which could recognize and detect mandelic acid (MA) enantiomers. Differential pulse voltammetry, quartz crystal microbalance, ultraviolet-visible spectroscopy, and atomic force microscopy were used to characterize the enantioselectivity. The results exhibited that γ-globulin modified electrode could enantioselectively recognize MA enantiomers, and larger response signals were obtained from R-MA. The factors influencing the performance of the resulting biosensor were investigated. The enantiomeric composition of R- and S-MA enantiomer mixtures could be determined by measuring the current responses of the sample. The developed electrodes have the advantages of simple preparation, good stability, and rapid detection.
一种新型的手性生物传感器已经通过将γ-球蛋白固定在修饰有金纳米粒子的玻碳电极上制备出来，它可以识别和检测扁桃酸（MA）对映异构体。差分脉冲伏安法、石英晶体微天平、紫外-可见光谱和原子力显微镜被用于表征对映选择性。结果表明，γ-球蛋白修饰电极可以对 MA 对映异构体进行选择性识别，并且从 R-MA 获得了更大的响应信号。研究了影响所得生物传感器性能的因素。通过测量样品的电流响应，可以确定 R-和 S-MA 对映体混合物的对映体组成。所开发的电极具有制备简单、稳定性好和检测快速的优点。
