手性立体选择伏安生物传感器用于肌醇和 D-手性肌醇的识别。

Stereoselective Voltammetric Biosensor for Myo-Inositol and D-Chiro-Inositol Recognition.

机构信息

Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.

Department of Chemistry and Drug Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.

出版信息

Sensors (Basel). 2023 Nov 16;23(22):9211. doi: 10.3390/s23229211.

DOI:10.3390/s23229211

PMID:38005597

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10674735/

Abstract

This paper describes the development of a simple voltammetric biosensor for the stereoselective discrimination of myo-inositol (myo-Ins) and D-chiro-inositol (D-chiro-Ins) by means of bovine serum albumin (BSA) adsorption onto a multi-walled carbon nanotube (MWCNT) graphite screen-printed electrode (MWCNT-GSPE), previously functionalized by the electropolymerization of methylene blue (MB). After a morphological characterization, the enantioselective biosensor platform was electrochemically characterized after each modification step by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The results show that the binding affinity between myo-Ins and BSA was higher than that between D-chiro-Ins and BSA, confirming the different interactions exhibited by the novel BSA/MB/MWCNT/GSPE platform towards the two diastereoisomers. The biosensor showed a linear response towards both stereoisomers in the range of 2-100 μM, with LODs of 0.5 and 1 μM for myo-Ins and D-chiro-Ins, respectively. Moreover, a stereoselectivity coefficient α of 1.6 was found, with association constants of 0.90 and 0.79, for the two stereoisomers, respectively. Lastly, the proposed biosensor allowed for the determination of the stereoisomeric composition of myo-/D-chiro-Ins mixtures in commercial pharmaceutical preparations, and thus, it is expected to be successfully applied in the chiral analysis of pharmaceuticals and illicit drugs of forensic interest.

摘要

本文描述了一种简单的伏安生物传感器的发展，用于通过牛血清白蛋白（BSA）吸附到多壁碳纳米管（MWCNT）石墨丝网印刷电极（MWCNT-GSPE）上，对肌醇（myo-Ins）和 D-手性肌醇（D-chiro-Ins）进行立体选择性区分，MWCNT-GSPE 之前通过亚甲蓝（MB）的电聚合功能化。在进行形态特征描述后，通过差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）对每个修饰步骤后的手性生物传感器平台进行电化学表征。结果表明，myo-Ins 与 BSA 之间的结合亲和力高于 D-chiro-Ins 与 BSA 之间的结合亲和力，这证实了新型 BSA/MB/MWCNT/GSPE 平台对两种非对映异构体表现出不同的相互作用。该生物传感器对两种立体异构体在 2-100 μM 的范围内表现出线性响应，myo-Ins 和 D-chiro-Ins 的检出限分别为 0.5 和 1 μM。此外，发现立体选择性系数 α 为 1.6，两种立体异构体的结合常数分别为 0.90 和 0.79。最后，该生物传感器可用于测定商业药物制剂中 myo-/D-chiro-Ins 混合物的立体异构体组成，因此有望成功应用于药物和法医学感兴趣的非法药物的手性分析。