Li Ling, Fukunaga-Kalabis Mizuho, Herlyn Meenhard
Molecular and Cellular Oncogenesis Program, Melanoma Research Center, The Wistar Institute, Philadelphia, PA, USA.
Methods Mol Biol. 2012;806:15-29. doi: 10.1007/978-1-61779-367-7_2.
Human melanocytes have been extensively studied, but a melanocyte stem cell reservoir in glabrous skin has not yet been found. Human dermis contains cells that are nonpigmented but can differentiate to several different cell types. We have recently shown that multipotent dermal stem cells isolated from human neonatal foreskins are able to differentiate to multiple cell lineages, including pigmented melanocytes. The dermal stem cells grow as three-dimensional spheres in human embryonic stem cell medium and express some neural crest stem cell and embryonic stem cell markers. Melanocytes derived from dermal stem cells express melanocytic markers and act the same way as mature epidermal melanocytes. Dermal spheres, embedded in the reconstructed dermis consisting of collagen with fibroblasts, can migrate to the basement membrane, where they become pigmented in the same way as epidermal melanocytes suggesting that dermal stem cells can give rise to epidermal melanocytes.
人类黑素细胞已得到广泛研究，但无毛皮肤中的黑素细胞干细胞库尚未被发现。人真皮含有未着色但可分化为几种不同细胞类型的细胞。我们最近发现，从人类新生儿包皮中分离出的多能真皮干细胞能够分化为多种细胞谱系，包括有色素的黑素细胞。真皮干细胞在人胚胎干细胞培养基中呈三维球体生长，并表达一些神经嵴干细胞和胚胎干细胞标志物。源自真皮干细胞的黑素细胞表达黑素细胞标志物，其行为与成熟表皮黑素细胞相同。嵌入由胶原蛋白和成纤维细胞组成的重建真皮中的真皮球体可迁移至基底膜，在那里它们与表皮黑素细胞一样产生色素，这表明真皮干细胞可产生表皮黑素细胞。
