Li Ling, Fukunaga-Kalabis Mizuho, Herlyn Meenhard
The Wistar Institute, Philadelphia, PA, USA.
Methods Mol Biol. 2013;989:235-46. doi: 10.1007/978-1-62703-330-5_18.
Skin, as the body's largest organ, has been extensively used to study adult stem cells. Most previous skin-related studies have focused on stem cells isolated from hair follicles and from keratinocytes. Here we present a protocol to isolate multipotent neural crest stem-like dermis-derived stem cells (termed dermal stem cells or DSCs) from human neonatal foreskins. DSCs grow like neural spheres in human embryonic stem cell medium and gain the ability to self-renew and differentiate into several cell lineages including melanocytes, neuronal cells, Schwann cells, smooth muscle cells, adipocytes, and chondrocytes. These cells express neural crest stem cell markers (NGFRp75 and nestin) as well as an embryonic stem cell marker (OCT4).
皮肤作为人体最大的器官，已被广泛用于研究成体干细胞。此前大多数与皮肤相关的研究都集中在从毛囊和角质形成细胞中分离出的干细胞。在此，我们展示了一种从人类新生儿包皮中分离多能性神经嵴干细胞样真皮来源干细胞（称为真皮干细胞或DSCs）的方案。DSCs在人类胚胎干细胞培养基中像神经球一样生长，并获得自我更新和分化为多种细胞谱系的能力，包括黑素细胞、神经元细胞、雪旺细胞、平滑肌细胞、脂肪细胞和软骨细胞。这些细胞表达神经嵴干细胞标志物（NGFRp75和巢蛋白）以及一种胚胎干细胞标志物（OCT4）。
