Assakura M T, Mandelbaum F R
Serviço de Bioquímica, Instituto Butantan, São Paulo, Brasil.
Toxicon. 1990;28(6):734-6. doi: 10.1016/0041-0101(90)90265-9.
Moojeni protease A, a proteolytic enzyme isolated from the venom of Bothrops moojeni hydrolyzes human and rabbit IgGs. The resulted fragments retained the combining though not the precipitating power or the property to fix complement. Similar to papain, moojeni protease A releases directly Fab fragments from IgG. In contrast to papain, however, the enzyme does not require the presence of thiol compounds either for activation or for reduction of the disulphide inter-heavy chain bridges. On the contrary, moojeni protease A is a metalloenzyme inhibited in the presence of thiol compounds.
穆杰尼蛋白酶A是一种从穆杰尼矛头蝮蛇毒中分离出的蛋白水解酶，它能水解人及兔的免疫球蛋白G。产生的片段保留了结合能力，但失去了沉淀能力或固定补体的特性。与木瓜蛋白酶相似，穆杰尼蛋白酶A能直接从免疫球蛋白G释放出Fab片段。然而，与木瓜蛋白酶不同的是，该酶无论是激活还是还原重链间的二硫键桥，都不需要硫醇化合物的存在。相反，穆杰尼蛋白酶A是一种金属酶，在硫醇化合物存在时会受到抑制。
