解偶联剂引起细胞色素b-c1复合物构象变化的圆二色性证据。
Circular dichroic evidence for a conformational change in a cytochrome b--c1 complex by uncoupling agents.
作者信息
Reed J, Reed T A, Hess B
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1045-8. doi: 10.1073/pnas.76.3.1045.
Abstract
Circular dichroic spectra of the cytochrome b--c1 complex exhibit bilobe formation typical of exciton splitting in the presence of uncoupler. Bilobe formation occurs if both cytochrome c1 and cytochrome b are fully reduced. The fully oxidized and ascorbate-reduced complexes are not altered dichroically by uncouplers. The exciton splitting induced by uncoupler is consistent with heme--heme interaction: specifically, interaction between the two cytochromes b in the complex.
摘要
细胞色素b-c1复合物的圆二色光谱在存在解偶联剂的情况下呈现出典型的激子分裂双峰形成。如果细胞色素c1和细胞色素b都完全还原，就会出现双峰形成。完全氧化和抗坏血酸还原的复合物不会因解偶联剂而发生二色性改变。解偶联剂诱导的激子分裂与血红素-血红素相互作用一致：具体来说，是复合物中两个细胞色素b之间的相互作用。
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J Am Chem Soc. 1967 Sep 27;89(20):5276-83. doi: 10.1021/ja00996a034.
2
Model systems for interacting heme moieties. I. The heme undecapeptide of cytochrome c.相互作用血红素部分的模型系统。I. 细胞色素c的血红素十一肽。
J Am Chem Soc. 1967 Aug 2;89(16):4190-6. doi: 10.1021/ja00992a601.
3
Stereochemistry of cooperative effects in haemoglobin.血红蛋白协同效应的立体化学
Nature. 1970 Nov 21;228(5273):726-39. doi: 10.1038/228726a0.
4
Protein-heme interactions in heme-proteins: cytochrome C.血红素蛋白中的蛋白质-血红素相互作用：细胞色素C
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5
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6
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