Cheong H S, Yoon D, Kim L H, Park B L, Lee H W, Han C S, Kim E M, Cho H, Chung E R, Cheong I, Shin H D
Department of Genetic Epidemiology, SNP Genetics, Inc., Rm 1407, Complex B, WooLim Lion's Valley, 371-28, Gasan-Dong, Geumcheon-Gu, Seoul, 153-803, Republic of Korea.
Meat Sci. 2007 Oct;77(2):257-63. doi: 10.1016/j.meatsci.2007.03.014. Epub 2007 Mar 24.
Marbling score (MS) is the major qualitative trait that affects carcass quality in beef cattle. In this study, we examined the association between genetic polymorphisms of the titin-cap gene (TCAP) and carcass traits in Korean native cattle (also known as Hanwoo). By direct DNA sequencing in 24 unrelated Korean cattle, we identified five sequence variants in 1.2kb of TCAP. Among them, four common polymorphic sites were selected for genotyping in the beef cattle (n=437). Pair-wise linkage analysis with four polymorphisms showed strong linkage disequilibrium (LD), and three major haplotypes (freq.>0.1) were constructed. Statistical analysis revealed that polymorphisms in intron1 (g.346G>A) and exon2 (g.592-597CTGCAG[Leu-Gln]insdel) showed significant association with marbling score (P(cor.)=0.003 and 0.02, respectively). One haplotype, ht2[C-G-G-del], also showed significant association with MS (P(cor.)=0.0004). Our findings suggest that polymorphisms in TCAP might be among the important genetic factors involved in carcass quality in beef cattle.
大理石花纹评分（MS）是影响肉牛胴体品质的主要质量性状。在本研究中，我们检测了肌联蛋白帽基因（TCAP）的遗传多态性与韩牛（也称为韩国本土牛）胴体性状之间的关联。通过对24头无亲缘关系的韩国牛进行直接DNA测序，我们在1.2kb的TCAP中鉴定出5个序列变异。其中，选择了4个常见多态性位点对肉牛（n = 437）进行基因分型。对4个多态性位点进行的成对连锁分析显示出强连锁不平衡（LD），并构建了3个主要单倍型（频率>0.1）。统计分析表明，内含子1（g.346G>A）和外显子2（g.592 - 597CTGCAG[Leu - Gln]插入/缺失）中的多态性与大理石花纹评分显著相关（校正P值分别为0.003和0.02）。一种单倍型ht2[C - G - G - del]也与MS显著相关（校正P值 = 0.0004）。我们的研究结果表明，TCAP中的多态性可能是参与肉牛胴体品质的重要遗传因素之一。
