Devine Carrick E, Payne Steven R, Peachey Bridget M, Lowe Timothy E, Ingram John R, Cook Christian J
Technology Development Group, HortResearch PB 3213, Hamilton, New Zealand.
Meat Sci. 2002 Feb;60(2):141-6. doi: 10.1016/s0309-1740(01)00115-2.
Immediately after electrical stimulation, the paired m. longissimus thoracis et lumborum (LT) of 40 sheep were boned out and wrapped tightly with a polyethylene cling film. One of the paired LT's was chilled in 15°C air to reach a rigor mortis (rigor) temperature of 18°C and the other side was placed in a water bath at 35°C and achieved rigor at this temperature. Wrapping reduced rigor shortening and mimicked meat left on the carcass. After rigor, the meat was aged at 15°C for 0, 8, 26 and 72 h and then frozen. The frozen meat was cooked to 75°C in an 85°C water bath and shear force values obtained from a 1×1 cm cross-section. The shear force values of meat for 18 and 35°C rigor were similar at zero ageing, but as ageing progressed, the 18 rigor meat aged faster and became more tender than meat that went into rigor at 35°C (P<0.001). The mean sarcomere length values of meat samples for 18 and 35°C rigor at each ageing time were significantly different (P<0.001), the samples at 35°C being shorter. When the short sarcomere length values and corresponding shear force values were removed for further data analysis, the shear force values for the 35°C rigor were still significantly greater. Thus the toughness of 35°C meat was not a consequence of muscle shortening and appears to be due to both a faster rate of tenderisation and the meat tenderising to a greater extent at the lower temperature. The cook loss at 35°C rigor (30.5%) was greater than that at 18°C rigor (28.4%) (P<0.01) and the colour Hunter L values were higher at 35°C (P<0.01) compared with 18°C, but there were no significant differences in a or b values.
电刺激后,立即取出40只绵羊的双侧胸腰最长肌(LT),剔骨后用聚乙烯保鲜膜紧密包裹。将其中一侧的LT在15°C空气中冷却,使其达到18°C的尸僵温度,另一侧置于35°C的水浴中并在此温度下达到尸僵。包裹减少了尸僵缩短,并模拟了留在胴体上的肉。尸僵后,肉在15°C下熟化0、8、26和72小时,然后冷冻。将冷冻肉在85°C水浴中煮至75°C,从1×1厘米的横截面获取剪切力值。在零熟化时,18°C和35°C尸僵肉的剪切力值相似,但随着熟化时间的延长,18°C尸僵肉熟化更快,比35°C尸僵的肉更嫩(P<0.001)。每个熟化时间下,18°C和35°C尸僵肉样的平均肌节长度值显著不同(P<0.001),35°C的样本较短。去除短肌节长度值和相应的剪切力值以进行进一步数据分析后,35°C尸僵肉的剪切力值仍然显著更高。因此,35°C肉的韧性不是肌肉缩短的结果,似乎是由于嫩化速度更快以及肉在较低温度下嫩化程度更大。35°C尸僵肉的煮损失率(30.5%)高于18°C尸僵肉(28.4%)(P<0.01),与18°C相比,35°C时的颜色亨特L值更高(P<0.01),但a值或b值没有显著差异。