Girish P S, Anjaneyulu A S R, Viswas K N, Shivakumar B M, Anand M, Patel M, Sharma B
Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India.
Meat Sci. 2005 May;70(1):107-12. doi: 10.1016/j.meatsci.2004.12.004.
Adulteration of high quality meat and meat products with their inferior/cheaper counterparts is a problem in the meat industry. The present study investigated the use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial 12S rRNA gene for identification of the origin of meats. PCR-RFLP was applied for species identification of beef, buffalo meat, mutton and chevon. PCR amplification yielded a 456-bp fragment in each of these species. The amplicons were digested with AluI, HhaI, ApoI and BspTI restriction enzymes resulting in a pattern that could identify and differentiate each of the above species. This technique did not yield satisfactory results with meat mixtures/meats. However, consistent results were obtained with both fresh and processed meat samples.
用劣质/廉价肉类及肉类产品掺假优质肉类及肉类产品是肉类行业存在的一个问题。本研究调查了利用线粒体12S rRNA基因的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）来鉴定肉类来源。PCR-RFLP被用于牛肉、水牛肉、羊肉和山羊肉的物种鉴定。PCR扩增在这些物种中均产生了一个456bp的片段。扩增产物用AluI、HhaI、ApoI和BspTI限制性内切酶进行消化，产生的图谱可用于鉴定和区分上述各物种。该技术对混合肉类/肉类未产生满意结果。然而，新鲜和加工肉类样品均获得了一致的结果。
