Zhang Yuli, Li Juan, Yang Shiqing, Xu Chao, Gblinwon Roselyn Tehzee, Hu Jianhui, Sun Xiaoxiang, Xia Guohua, Yang Huan, Shen Yuping
School of Pharmacy, Jiangsu University, Zhenjiang, China.
Department of Pharmacy, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang, China.
Mitochondrial DNA B Resour. 2021 Mar 19;6(3):1044-1049. doi: 10.1080/23802359.2021.1899075.
A triplex PCR assay was developed to identify animal species and adulteration of a natural medicine (GGEC). Three species-specific primer sets were designed according to the difference in mitochondrial genome of , and . The PCR conditions were optimized and the assay was well validated for high specificity and sensitivity (1 mg/μL). Especially, when artificial adulterants made from the mixture of three species were analyzed, the assay has still exhibited strong capability of differentiation. By using this developed method, two batches out of fourteen commercial GGEC products were identified to be adulterated by . The newly proposed assay showed sufficient merits as a regular tool for the identification of counterfeits or adulterants of GGEC product for their pulverized and processed form, and even Chinese patent medicines composed of these species.
开发了一种三重PCR检测方法，用于鉴定动物物种以及一种天然药物（GGEC）的掺假情况。根据[物种1]、[物种2]和[物种3]线粒体基因组的差异设计了三套物种特异性引物。优化了PCR条件，并对该检测方法的高特异性和灵敏度（1 mg/μL）进行了充分验证。特别是，当分析由这三个物种混合制成的人工掺假物时，该检测方法仍表现出很强的区分能力。通过使用这种开发的方法，在14批市售GGEC产品中，有两批被鉴定为被[掺假物种]掺假。新提出的检测方法作为一种常规工具，对于鉴定GGEC产品及其粉碎和加工形式甚至由这些物种组成的中成药的假冒或掺假情况具有足够的优势。
