Department of Ophthalmology, University of Colorado Health and Science Center, Rocky Mountain Lions Eye Institute, University of Colorado School of Medicine, Aurora, Colorado, USA.
Invest Ophthalmol Vis Sci. 2012 Jan 25;53(1):354-60. doi: 10.1167/iovs.11-8496.
To evaluate the tolerance and biocompatibility of a preformed nitinol intraocular clip in an animal model after anterior segment surgery.
Yucatan mini-pigs were used. A 30-gauge prototype injector was used to attach a shape memory nitinol clip to the iris of five pigs. Another five eyes received conventional polypropylene suture with a modified Seipser slip knot. The authors compared the surgical time of each technique. All eyes underwent standard full-field electroretinogram at baseline and 8 weeks after surgery. The animals were euthanized and eyes collected for histologic analysis after 70 days (10 weeks) postsurgery. The corneal thickness, corneal endothelial cell counts, specular microscopy parameters, retina cell counts, and electroretinogram parameters were compared between the groups. A two sample t-test for means and a P value of 0.05 were use for assessing statistical differences between measurements.
The injection of the nitinol clip was 15 times faster than conventional suturing. There were no statistical differences between the groups for corneal thickness, endothelial cell counts, specular microscopy parameters, retina cell counts, and electroretinogram measurements.
The nitinol clip prototype is well tolerated and showed no evidence of toxicity in the short-term. The injectable delivery system was faster and technically less challenging than conventional suture techniques.
评估前节手术后动物模型中预制形状记忆镍钛诺眼内夹的耐受性和生物相容性。
使用尤卡坦小型猪。使用 30 号探针注射器将形状记忆镍钛诺夹固定在五只猪的虹膜上。另外五只眼睛接受常规聚丙烯缝线和改良的 Seipser 滑结。作者比较了两种技术的手术时间。所有眼睛在基线和手术后 8 周均进行标准全视野视网膜电图检查。术后 70 天（10 周）处死动物并收集眼睛进行组织学分析。比较各组之间的角膜厚度、角膜内皮细胞计数、共焦显微镜参数、视网膜细胞计数和视网膜电图参数。采用均值的两样本 t 检验和 P 值为 0.05 用于评估测量值之间的统计学差异。
与传统缝合相比，镍钛诺夹的注射速度快 15 倍。两组间的角膜厚度、内皮细胞计数、共焦显微镜参数、视网膜细胞计数和视网膜电图测量值均无统计学差异。
在短期内，镍钛诺夹原型具有良好的耐受性，没有毒性证据。与传统缝线技术相比，可注射输送系统更快，技术难度更低。
