Denhez F, Lafyatis R, Kondaiah P, Roberts A B, Sporn M B
Laboratory of Chemoprevention, National Institute of Health, Bethesda, Maryland 20892.
Growth Factors. 1990;3(2):139-46. doi: 10.3109/08977199009108276.
Two TGF-beta s, TGF-beta 1 and 2, have previously been isolated from the mouse. Here we report the isolation of a murine TGF-beta 3 cDNA. RNAs extracted from 15-day-old mouse embryos and several mouse cell lines were reverse-transcribed. These cDNA mixtures were used as substrates for polymerase chain-reaction amplifications, using oligonucleotides designed on the basis of known human and chicken TGF-beta 3 sequences, including the initiation and stop codons. Several overlapping cDNAs containing either the amino-terminal domain or the carboxy-terminal domain, as well as the complete 1.2-kb coding region of the mouse TGF-beta 3 cDNA were obtained. The mouse TGF-beta 3 coding region is 1230 nucleotides long and codes for a 410 amino acid polypeptide very similar to its human counterpart. This cDNA hybridizes to a unique 3.5-kb RNA and is differentially expressed in various mouse tissues and at different embryonic stages.
此前已从小鼠中分离出两种转化生长因子β（TGF-β），即TGF-β1和TGF-β2。在此，我们报告小鼠TGF-β3 cDNA的分离情况。从15日龄小鼠胚胎和几种小鼠细胞系中提取的RNA进行了逆转录。这些cDNA混合物用作聚合酶链反应扩增的底物，使用基于已知人类和鸡TGF-β3序列（包括起始密码子和终止密码子）设计的寡核苷酸。获得了几个包含氨基末端结构域或羧基末端结构域的重叠cDNA，以及小鼠TGF-β3 cDNA完整的1.2 kb编码区。小鼠TGF-β3编码区长1230个核苷酸，编码一个410个氨基酸的多肽，与人类对应物非常相似。该cDNA与一条独特的3.5 kb RNA杂交，并在小鼠的各种组织和不同胚胎阶段差异表达。
