Yamazoe Hironori
Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
J Biomed Mater Res A. 2012 Feb;100(2):362-9. doi: 10.1002/jbm.a.33279. Epub 2011 Nov 7.
A functional substrate capable of regulating protein adsorption was prepared using a crosslinked albumin (cl-albumin) film for use in the fabrication of protein micropatterns. The adsorption of proteins with different characteristics onto cl-albumin film, including serum proteins, serum albumin, and lysozyme, was investigated using a quartz crystal microbalance. The results showed that surfaces coated with cl-albumin film are highly resistant to protein adsorption, regardless of protein charge and rigidity. In addition, this adsorption-resistance property can be easily converted to promote protein adsorption by exposing the cl-albumin film to a charged polymer solution. By combining the convertible surface property of cl-albumin film and inkjet printing techniques, a precise protein micropattern was successfully fabricated on the substrate. Protein adsorption onto the wall surface of microchannels could also be suppressed or promoted by coating the surface with cl-albumin film. This approach will aid in the development of biomaterials carrying protein micropatterns, such as biosensors, biochips, and cellular scaffolds.
采用交联白蛋白（cl-白蛋白）膜制备了一种能够调节蛋白质吸附的功能性底物，用于蛋白质微图案的制备。使用石英晶体微天平研究了包括血清蛋白、血清白蛋白和溶菌酶在内的不同特性蛋白质在cl-白蛋白膜上的吸附情况。结果表明，无论蛋白质的电荷和刚性如何，涂有cl-白蛋白膜的表面对蛋白质吸附具有高度抗性。此外，通过将cl-白蛋白膜暴露于带电聚合物溶液中，这种抗吸附特性可以很容易地转变为促进蛋白质吸附。通过结合cl-白蛋白膜的可转换表面特性和喷墨打印技术，在底物上成功制备了精确的蛋白质微图案。通过用cl-白蛋白膜涂覆微通道壁表面，也可以抑制或促进蛋白质在微通道壁表面的吸附。这种方法将有助于开发携带蛋白质微图案的生物材料，如生物传感器、生物芯片和细胞支架。
