Ren Jiang-Jian, Xu Jian-Zhong, Yu Xu-Ping
Zhejiang Institute of Traditional Chinese Medicine, Hangzhou 310023, China.
Zhong Yao Cai. 2009 Jul;32(7):1026-8.
To choose the best harvest time and initial processing method of Rhizoma Corydalis.
Taking the content of tetrahydropalmatine and dry rates as major indices, different samples from different harvest time and different processing methods were investigated by HPLC. The C18 column (250 mm x 4.6 mm, 5 microm) was used with mobile phase of methanol-0.1% phosphoric acid solution (pH = 6.0, adjusted with triethylamine) (58: 42). The mobile phase flow rate was 1.0 mL/min. The detection wavelength was 280 nm.
The contents of tetrahydropalmatine were similar although the harvest time was different. The dry rate on May 21 was higher than the others. The content of tetrahydropalmatine was the highest by steaming and reached 0.11%.
The best harvest time for Rhizoma Corydalis is around 10 days after the above-ground plant wilted, and the best initial processing method for Rhizoma Corydalis is steaming.
筛选延胡索的最佳采收期和初加工方法。
以延胡索中延胡索乙素含量和干燥率为主要指标,采用高效液相色谱法对不同采收期和不同加工方法的延胡索样品进行考察。采用C18柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸溶液(pH = 6.0,用三乙胺调节)(58∶42)为流动相。流动相流速为1.0 mL/min。检测波长为280 nm。
不同采收期延胡索乙素含量相近,5月21日干燥率高于其他日期。蒸制后延胡索乙素含量最高,达0.11%。
延胡索最佳采收期为地上植株枯萎后10 d左右,最佳初加工方法为蒸制。