[延胡索、破壁延胡索及其炮制品有效成分的研究]

[Studies on active ingredients in Corydalis, broken cell wall corydalis and its processed products].

作者信息

Cao Liu, Diu Zhi-Ying, Wang Ping, Sun Wei, Tian Yong-Liang

机构信息

Tianjin University of Traditional Chinese Medicine University, Tianjin 300193, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Jun;33(11):1263-5.

PMID:18831201

Abstract

OBJECTIVE

To compare the contents of tetrahydropalmatine and dehydrocorydaline in corydalis, broken cell wall corydalis and its different processed products.

METHOD

The broken cell wall technique was used to corydalis, and then both the corydalis and broken cell wall corydalis were processed. The method of chromatography which was used to determine the contents of tetrahydropalmatine and dehydrocorydaline in corydalis broken cell wall corydalis and its different processed products was performed by RP-HPLC with Kromasil ODS-C18 (4.6 mm x 250 mm, 5 microm) column was used at 35 degrees C, acetonitrile-acetate buffer solution (pH 6.0) (30:70) as mobile phase of 1 mL x min(-1) flow rate, detection wavelength was set at 280 nm.

RESULT

The contents of the two active components in broken cell wall corydalis were higher than that in corydalis, while that in broken cell wall and vinegar-fried corydalis was highest in the products of breaked cell wall corydalis.

CONCLUSION

Breaking the cell wall of corydalis can help to dissolute alkaloids.

摘要

目的

比较延胡索、破壁延胡索及其不同炮制品中延胡索乙素和去氢紫堇碱的含量。

方法

采用破壁技术处理延胡索，然后对延胡索和破壁延胡索进行炮制。采用RP-HPLC法测定延胡索、破壁延胡索及其不同炮制品中延胡索乙素和去氢紫堇碱的含量，色谱柱为Kromasil ODS-C18（4.6 mm×250 mm，5μm），柱温35℃，以乙腈-醋酸缓冲液（pH 6.0）（30:70）为流动相，流速为1 mL·min⁻¹，检测波长为280 nm。