Faculty of Marine Bioscience & Technology, Gangneung-Wonju National University, Gangneung, 210-702, Republic of Korea.
J Microbiol. 2011 Oct;49(5):741-6. doi: 10.1007/s12275-011-1209-8. Epub 2011 Nov 9.
The viral diseases have been the serious problem in salmonid farming, and rainbow trout is not an exception. In this study, routine surveys were conducted for detecting of viruses in farmed rainbow trout (Oncorhynchus mykiss) in Korea during 2009-2010. Head kidneys from individual fish were employed for virus detection by using a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) were the target viruses in this study. 53.5% (46/86) were found to be IPNV-positive, while IHNV and VHSV showed RT-LAMP negative during examination for 2 years. Ten IPNV-positive samples were randomly selected for viral isolation and the cells showing CPEs were subjected to RT-LAMP, RT-PCR, and direct sequencing. Phylogenetic analysis showed that the rainbow trout isolate has high similarity homologies with the VR-299 strain, as previously described.
病毒性疾病一直是鲑鱼养殖中的严重问题，虹鳟鱼也不例外。在这项研究中，对 2009 年至 2010 年韩国养殖虹鳟鱼中的病毒进行了常规检测。使用反转录环介导等温扩增（RT-LAMP）检测法，从个体鱼的头肾中检测病毒。本研究的靶病毒为传染性胰脏坏死病毒（IPNV）、传染性造血器官坏死病毒（IHNV）和病毒性出血性败血症病毒（VHSV）。结果发现，86 份样本中有 53.5%（46/86）为 IPNV 阳性，而在 2 年的检测中，IHNV 和 VHSV 均呈 RT-LAMP 阴性。随机选择了 10 个 IPNV 阳性样本进行病毒分离，出现 CPE 的细胞进行 RT-LAMP、RT-PCR 和直接测序。系统进化分析表明，虹鳟鱼分离株与之前描述的 VR-299 株具有高度相似的同源性。
